FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039313701> ?p ?o ?g. }

• W2039313701 endingPage "4083" @default.
• W2039313701 startingPage "4083" @default.
• W2039313701 abstract "Both the -366/+43 and the -282/+43 mouse alphaA-crystallin (or alphaA) promoters have been effective at driving transgene expression in lens fiber cells, but not in lens epithelium. Because the chick delta1-crystallin gene is expressed in lens epithelial cells, an enhancer was borrowed from this gene and linked to the alphaA promoter. This heterogenic enhancer/promoter construct was tested in transgenic mice to see whether it was active in both lens epithelium and fiber cells while retaining lens specificity.The third intron of the chick delta1-crystallin gene, which contains a lens enhancer element, was added to the 5' end of the mouse alphaA promoter. We refer to this chimeric regulatory element as the deltaenalphaA promoter. To test its activity, we inserted coding sequences for five different genes. Transgenic mice were generated by pronuclear microinjection. Transgene expression patterns were analyzed by either X-gal staining, in situ hybridization or immunohistochemical staining.When deltaenalphaA-lacZ transgenic embryos were stained with X-gal at embryonic day (E)11.5, beta-galactosidase activity was detected only in the eye. Histologic sections of the stained embryos revealed that lacZ was expressed exclusively in the lens, in both epithelial and fiber cells. Transgenic mice were also generated using either the original alphaA- or the new deltaenalphaA promoter linked to an insulin cDNA. In situ hybridizations confirmed that the short alphaA promoter targeted prenatal insulin expression specifically to the lens fiber cells, whereas the deltaenalphaA promoter was active in both lens epithelial and fiber cells. Developmental studies of the deltaenalphaA-insulin mice showed that the deltaenalphaA promoter became active at the lens pit stage and remained active in all lens cells, even at postnatal ages. The deltaenalphaA promoter also successfully directed expression of SV40 T-antigen (TAg), human E2F2, and dominant negative Sprouty2 (dn-Spry2) genes to lens epithelial and fiber cells. The lens specificity of the deltaenalphaA promoter was maintained in minigenes with different types of introns and polyadenylation signals.A new lens-specific regulatory element was generated-the deltaenalphaA promoter, which can drive high levels of transgene expression in both lens epithelium and fiber cells throughout development. This modified promoter can be used for future transgenic studies of signal transduction and cell cycle regulation in lens epithelial cells." @default.
• W2039313701 created "2016-06-24" @default.
• W2039313701 creator A5025137671 @default.
• W2039313701 creator A5026882014 @default.
• W2039313701 creator A5038793367 @default.
• W2039313701 creator A5040829995 @default.
• W2039313701 creator A5041007486 @default.
• W2039313701 creator A5091698523 @default.
• W2039313701 date "2004-11-01" @default.
• W2039313701 modified "2023-09-23" @default.
• W2039313701 title "Chick δ1-Crystallin Enhancer Influences Mouse αA-Crystallin Promoter Activity in Transgenic Mice" @default.
• W2039313701 cites W1262514376 @default.
• W2039313701 cites W1559767592 @default.
• W2039313701 cites W1560117034 @default.
• W2039313701 cites W1608891270 @default.
• W2039313701 cites W1620706982 @default.
• W2039313701 cites W169817699 @default.
• W2039313701 cites W1789601839 @default.
• W2039313701 cites W1829274477 @default.
• W2039313701 cites W1925182101 @default.
• W2039313701 cites W1933017411 @default.
• W2039313701 cites W1973955621 @default.
• W2039313701 cites W1980513503 @default.
• W2039313701 cites W1982511318 @default.
• W2039313701 cites W1991498195 @default.
• W2039313701 cites W2000106017 @default.
• W2039313701 cites W2001074620 @default.
• W2039313701 cites W2002059595 @default.
• W2039313701 cites W2002609010 @default.
• W2039313701 cites W2002996311 @default.
• W2039313701 cites W2009578263 @default.
• W2039313701 cites W2014409793 @default.
• W2039313701 cites W2020292268 @default.
• W2039313701 cites W2028453954 @default.
• W2039313701 cites W2031715332 @default.
• W2039313701 cites W2035184168 @default.
• W2039313701 cites W2042339641 @default.
• W2039313701 cites W2043218606 @default.
• W2039313701 cites W2053428527 @default.
• W2039313701 cites W2062519756 @default.
• W2039313701 cites W2071957913 @default.
• W2039313701 cites W2074610333 @default.
• W2039313701 cites W2077549378 @default.
• W2039313701 cites W2078872477 @default.
• W2039313701 cites W2094555201 @default.
• W2039313701 cites W2108317548 @default.
• W2039313701 cites W2112718652 @default.
• W2039313701 cites W2130993929 @default.
• W2039313701 cites W2131888998 @default.
• W2039313701 cites W2132049966 @default.
• W2039313701 cites W2135338236 @default.
• W2039313701 cites W2135445817 @default.
• W2039313701 cites W2140894105 @default.
• W2039313701 cites W2147395274 @default.
• W2039313701 cites W2161889215 @default.
• W2039313701 cites W2161999936 @default.
• W2039313701 cites W2169913534 @default.
• W2039313701 cites W2211576543 @default.
• W2039313701 cites W2253951324 @default.
• W2039313701 cites W2337257182 @default.
• W2039313701 cites W2345066775 @default.
• W2039313701 cites W335477860 @default.
• W2039313701 cites W38543926 @default.
• W2039313701 doi "https://doi.org/10.1167/iovs.03-1270" @default.
• W2039313701 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15505059" @default.
• W2039313701 hasPublicationYear "2004" @default.
• W2039313701 type Work @default.
• W2039313701 sameAs 2039313701 @default.
• W2039313701 citedByCount "30" @default.
• W2039313701 countsByYear W20393137012012 @default.
• W2039313701 countsByYear W20393137012014 @default.
• W2039313701 countsByYear W20393137012015 @default.
• W2039313701 countsByYear W20393137012017 @default.
• W2039313701 countsByYear W20393137012019 @default.
• W2039313701 countsByYear W20393137012021 @default.
• W2039313701 countsByYear W20393137012023 @default.
• W2039313701 crossrefType "journal-article" @default.
• W2039313701 hasAuthorship W2039313701A5025137671 @default.
• W2039313701 hasAuthorship W2039313701A5026882014 @default.
• W2039313701 hasAuthorship W2039313701A5038793367 @default.
• W2039313701 hasAuthorship W2039313701A5040829995 @default.
• W2039313701 hasAuthorship W2039313701A5041007486 @default.
• W2039313701 hasAuthorship W2039313701A5091698523 @default.
• W2039313701 hasConcept C101762097 @default.
• W2039313701 hasConcept C102230213 @default.
• W2039313701 hasConcept C104317684 @default.
• W2039313701 hasConcept C111936080 @default.
• W2039313701 hasConcept C141035611 @default.
• W2039313701 hasConcept C150194340 @default.
• W2039313701 hasConcept C151730666 @default.
• W2039313701 hasConcept C15336307 @default.
• W2039313701 hasConcept C153911025 @default.
• W2039313701 hasConcept C2909221355 @default.
• W2039313701 hasConcept C54355233 @default.
• W2039313701 hasConcept C62525196 @default.
• W2039313701 hasConcept C7263354 @default.
• W2039313701 hasConcept C81439078 @default.
• W2039313701 hasConcept C86803240 @default.

• next