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- W2039317811 abstract "Angiotensin I (ANG I) from various nonmammalian vertebrates does not bind the antibody to human ANG I, and thus radioimmunoassay (RIA) is not applicable for measurement of renin activity. It is largely due to this difficulty that biochemistry and physiology of the renin-angiotensin system of nonmammalian vertebrates have not been studied. The present work was therefore undertaken to develop a system to determine plasma renin activity by measuring the ANG I generation rate with RIA in fowl plasma. Antibody to [Asp1,Val5,Ser9]-angiotensin I (fowl ANG I) was produced by immunizing goats and rabbits with a conjugate of human immunoglobulin and fowl ANG I. An antiserum, with a maximum binding of 86.3%, was obtained from the goat after six intradermal injections of the conjugate (each contained 0.25 mg fowl ANG I). This antiserum (at a final dilution of 1:200,000), and 125I-labeled fowl ANG I, purified by DEAE-cellulose and Sephadex G-25 column chromatography, were used for the RIA of natural fowl ANG I. Fowl ANG I generated by incubating plasma and kidney extract, was measured by both RIA and rat vasopressor assay. The values obtained from these two methods correlated well. The formation of angiotensin increased when the duration of incubation was prolonged, and was optimum at pH 5.0–5.5. Plasma renin activity (rate of fowl ANG I generation) in 50 μl plasma from anesthetized female white leghorn chickens (24 weeks, n = 6) was 15.04 ± 2.26 ng/ml·hr, and that in plasma from chronically cannulated conscious chickens (30 weeks, n = 5) was 4.58 ± 0.61 ng/ml·hr. The plasma angiotensinogen level was 500–600 ng/ml in both groups. Thus this sensitive RIA method may provide a useful tool to study the functions and regulation of the renin-angiotensin system in fowl." @default.
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- W2039317811 date "1981-10-01" @default.
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- W2039317811 title "Radioimmunoassay of fowl angiotensin I" @default.
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- W2039317811 doi "https://doi.org/10.1016/0016-6480(81)90112-x" @default.
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