FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039350651> ?p ?o ?g. }

• W2039350651 endingPage "e40433" @default.
• W2039350651 startingPage "e40433" @default.
• W2039350651 abstract "X-box binding protein-1 (XBP-1) is an important regulator of a subset of genes during endoplasmic reticulum (ER) stress. In the current study, we analyzed endogenous XBP-1 expression and localization, with a view to determining the effects of ER stress on the developmental competency of preimplantation embryos in mice. Fluorescence staining revealed that functional XBP-1 is localized on mature oocyte spindles and abundant in the nucleus at the germinal vesicle (GV) stage. However, in preimplantation embryos, XBP-1 was solely detected in the cytoplasm at the one-cell stage. The density of XBP-1 was higher in the nucleus than the cytoplasm at the two-cell, four-cell, eight-cell, morula, and blastocyst stages. Furthermore, RT-PCR analysis confirmed active XBP-1 mRNA splicing at all preimplantation embryo stages, except the one-cell stage. Tunicamycin (TM), an ER stress inducer used as a positive control, promoted an increase in the density of nuclear XBP-1 at the one-cell and two-cell stages. Similarly, culture medium supplemented with 25 mM sorbitol displayed a remarkable increase active XBP-1 expression in the nuclei of 1-cell and 2-cell embryos. Conversely, high concentrations of TM or sorbitol led to reduced nuclear XBP-1 density and significant ER stress-induced apoptosis. Tauroursodeoxycholic acid (TUDCA), a known inhibitor of ER stress, improved the rate of two-cell embryo development to blastocysts by attenuating the expression of active XBP-1 protein in the nucleus at the two-cell stage. Our data collectively suggest that endogenous XBP-1 plays a role in normal preimplantation embryonic development. Moreover, XBP-1 splicing is activated to generate a functional form in mouse preimplantation embryos during culture stress. TUDCA inhibits hyperosmolar-induced ER stress as well as ER stress-induced apoptosis during mouse preimplantation embryo development." @default.
• W2039350651 created "2016-06-24" @default.
• W2039350651 creator A5018055051 @default.
• W2039350651 creator A5050292482 @default.
• W2039350651 creator A5057821011 @default.
• W2039350651 creator A5078669547 @default.
• W2039350651 date "2012-07-13" @default.
• W2039350651 modified "2023-10-14" @default.
• W2039350651 title "Inhibition of Endoplasmic Reticulum Stress Improves Mouse Embryo Development" @default.
• W2039350651 cites W1672219033 @default.
• W2039350651 cites W1758816958 @default.
• W2039350651 cites W1965158573 @default.
• W2039350651 cites W1965843055 @default.
• W2039350651 cites W1968796668 @default.
• W2039350651 cites W1969117475 @default.
• W2039350651 cites W1970090817 @default.
• W2039350651 cites W1974968790 @default.
• W2039350651 cites W1991319495 @default.
• W2039350651 cites W1998562057 @default.
• W2039350651 cites W1998626286 @default.
• W2039350651 cites W2001399725 @default.
• W2039350651 cites W2014359260 @default.
• W2039350651 cites W2025846979 @default.
• W2039350651 cites W2027604252 @default.
• W2039350651 cites W2027727753 @default.
• W2039350651 cites W2034732491 @default.
• W2039350651 cites W2034810866 @default.
• W2039350651 cites W2035087026 @default.
• W2039350651 cites W2040651817 @default.
• W2039350651 cites W2048456744 @default.
• W2039350651 cites W2054128877 @default.
• W2039350651 cites W2062141110 @default.
• W2039350651 cites W2063193694 @default.
• W2039350651 cites W2070389094 @default.
• W2039350651 cites W2079944569 @default.
• W2039350651 cites W2080901528 @default.
• W2039350651 cites W2081971038 @default.
• W2039350651 cites W2083865116 @default.
• W2039350651 cites W2086165345 @default.
• W2039350651 cites W2086365629 @default.
• W2039350651 cites W2089302815 @default.
• W2039350651 cites W2094274061 @default.
• W2039350651 cites W2094908106 @default.
• W2039350651 cites W2106314483 @default.
• W2039350651 cites W2107100228 @default.
• W2039350651 cites W2107287236 @default.
• W2039350651 cites W2107601804 @default.
• W2039350651 cites W2111517707 @default.
• W2039350651 cites W2112286580 @default.
• W2039350651 cites W2122345674 @default.
• W2039350651 cites W2127906377 @default.
• W2039350651 cites W2128370636 @default.
• W2039350651 cites W2140277678 @default.
• W2039350651 cites W2141261198 @default.
• W2039350651 cites W2158940378 @default.
• W2039350651 cites W2159466552 @default.
• W2039350651 cites W2161083011 @default.
• W2039350651 cites W2163411496 @default.
• W2039350651 cites W2165064047 @default.
• W2039350651 cites W2165796896 @default.
• W2039350651 cites W2169048699 @default.
• W2039350651 doi "https://doi.org/10.1371/journal.pone.0040433" @default.
• W2039350651 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3396646" @default.
• W2039350651 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22808162" @default.
• W2039350651 hasPublicationYear "2012" @default.
• W2039350651 type Work @default.
• W2039350651 sameAs 2039350651 @default.
• W2039350651 citedByCount "71" @default.
• W2039350651 countsByYear W20393506512012 @default.
• W2039350651 countsByYear W20393506512013 @default.
• W2039350651 countsByYear W20393506512014 @default.
• W2039350651 countsByYear W20393506512015 @default.
• W2039350651 countsByYear W20393506512016 @default.
• W2039350651 countsByYear W20393506512017 @default.
• W2039350651 countsByYear W20393506512018 @default.
• W2039350651 countsByYear W20393506512019 @default.
• W2039350651 countsByYear W20393506512020 @default.
• W2039350651 countsByYear W20393506512021 @default.
• W2039350651 countsByYear W20393506512022 @default.
• W2039350651 countsByYear W20393506512023 @default.
• W2039350651 crossrefType "journal-article" @default.
• W2039350651 hasAuthorship W2039350651A5018055051 @default.
• W2039350651 hasAuthorship W2039350651A5050292482 @default.
• W2039350651 hasAuthorship W2039350651A5057821011 @default.
• W2039350651 hasAuthorship W2039350651A5078669547 @default.
• W2039350651 hasBestOaLocation W20393506511 @default.
• W2039350651 hasConcept C104317684 @default.
• W2039350651 hasConcept C139447449 @default.
• W2039350651 hasConcept C153911025 @default.
• W2039350651 hasConcept C158617107 @default.
• W2039350651 hasConcept C190062978 @default.
• W2039350651 hasConcept C196843134 @default.
• W2039350651 hasConcept C2777575526 @default.
• W2039350651 hasConcept C2778177303 @default.
• W2039350651 hasConcept C2778997996 @default.
• W2039350651 hasConcept C2781331208 @default.
• W2039350651 hasConcept C54458228 @default.
• W2039350651 hasConcept C55493867 @default.

• next