Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039366870> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2039366870 endingPage "265" @default.
- W2039366870 startingPage "253" @default.
- W2039366870 abstract "Monoclonal antibodies (mAbs) which recognize separate epitopes on ovine immunoglobulin E (IgE) have been used to develop a non-competitive antibody sandwich enzyme immunoassay (EIA) for quantitating ovine IgE. Purified anti-IgE mAb (YD3) coated onto polystyrene microtitre plates was used to capture IgE in serum samples. Biotinylated anti-IgE mAb (XB6) followed by streptavidin conjugated with horseradish peroxidase were used to detect captured IgE. Tetramethylbenzidine and H2O2 were used as enzyme substrate. A reference serum was prepared by pooling sheep sera containing elevated IgE levels. This reference serum was assigned a value of 100 units ml−1 and used to prepare standard curves for the EIA. The linear region of log-log transformed standard curve data covered a range of 0.05-0.8 units ml−. The equation of a linear regression line fitted to this curve was used to determine sample concentrations. Using purified IgE, 1 unit of reference serum was equivalent to 0.86 μg ml− IgE. Maximum intra- and inter-assay coefficients of variation for the EIA were 4.6% and 9.7%, respectively. Subjecting serum samples to 15 freeze/thaw cycles, storage at room temperature for 16 days or incubation at 37°C for 8 h resulted in minimal loss of IgE detection. Incubation of serum at 56°C resulted in rapid reduction in detection of IgE by the EIA. The assay was used to determine IgE levels in adult sheep monospecifically infected with weekly doses of the nematode Trichostrongylus axei. Serum IgE levels increased from 9 to 16 days following first infection and reached maximum levels by days 35–58. Serum IgE responses closely followed IgE positive cell responses in the abomasal mucosa." @default.
- W2039366870 created "2016-06-24" @default.
- W2039366870 creator A5005423147 @default.
- W2039366870 creator A5014870039 @default.
- W2039366870 creator A5068862688 @default.
- W2039366870 creator A5079882782 @default.
- W2039366870 date "1997-07-01" @default.
- W2039366870 modified "2023-09-23" @default.
- W2039366870 title "Quantification of total sheep IgE concentration using anti-ovine IgE monoclonal antibodies in an enzyme immunoassay" @default.
- W2039366870 cites W1969258362 @default.
- W2039366870 cites W1976485736 @default.
- W2039366870 cites W2005142640 @default.
- W2039366870 cites W2006726135 @default.
- W2039366870 cites W2019499716 @default.
- W2039366870 cites W2075754598 @default.
- W2039366870 cites W2077615471 @default.
- W2039366870 cites W2095169766 @default.
- W2039366870 cites W2095520922 @default.
- W2039366870 cites W2147269137 @default.
- W2039366870 cites W1964321286 @default.
- W2039366870 doi "https://doi.org/10.1016/s0165-2427(97)00010-x" @default.
- W2039366870 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9261963" @default.
- W2039366870 hasPublicationYear "1997" @default.
- W2039366870 type Work @default.
- W2039366870 sameAs 2039366870 @default.
- W2039366870 citedByCount "34" @default.
- W2039366870 countsByYear W20393668702012 @default.
- W2039366870 countsByYear W20393668702014 @default.
- W2039366870 countsByYear W20393668702019 @default.
- W2039366870 countsByYear W20393668702022 @default.
- W2039366870 crossrefType "journal-article" @default.
- W2039366870 hasAuthorship W2039366870A5005423147 @default.
- W2039366870 hasAuthorship W2039366870A5014870039 @default.
- W2039366870 hasAuthorship W2039366870A5068862688 @default.
- W2039366870 hasAuthorship W2039366870A5079882782 @default.
- W2039366870 hasConcept C141105273 @default.
- W2039366870 hasConcept C153911025 @default.
- W2039366870 hasConcept C159654299 @default.
- W2039366870 hasConcept C185592680 @default.
- W2039366870 hasConcept C203014093 @default.
- W2039366870 hasConcept C204628709 @default.
- W2039366870 hasConcept C2780420688 @default.
- W2039366870 hasConcept C43617362 @default.
- W2039366870 hasConcept C542903549 @default.
- W2039366870 hasConcept C70784841 @default.
- W2039366870 hasConcept C86803240 @default.
- W2039366870 hasConceptScore W2039366870C141105273 @default.
- W2039366870 hasConceptScore W2039366870C153911025 @default.
- W2039366870 hasConceptScore W2039366870C159654299 @default.
- W2039366870 hasConceptScore W2039366870C185592680 @default.
- W2039366870 hasConceptScore W2039366870C203014093 @default.
- W2039366870 hasConceptScore W2039366870C204628709 @default.
- W2039366870 hasConceptScore W2039366870C2780420688 @default.
- W2039366870 hasConceptScore W2039366870C43617362 @default.
- W2039366870 hasConceptScore W2039366870C542903549 @default.
- W2039366870 hasConceptScore W2039366870C70784841 @default.
- W2039366870 hasConceptScore W2039366870C86803240 @default.
- W2039366870 hasIssue "3-4" @default.
- W2039366870 hasLocation W20393668701 @default.
- W2039366870 hasLocation W20393668702 @default.
- W2039366870 hasOpenAccess W2039366870 @default.
- W2039366870 hasPrimaryLocation W20393668701 @default.
- W2039366870 hasRelatedWork W1989542447 @default.
- W2039366870 hasRelatedWork W1995724549 @default.
- W2039366870 hasRelatedWork W1996001026 @default.
- W2039366870 hasRelatedWork W2039366870 @default.
- W2039366870 hasRelatedWork W2059392690 @default.
- W2039366870 hasRelatedWork W2080815938 @default.
- W2039366870 hasRelatedWork W2087982072 @default.
- W2039366870 hasRelatedWork W2368460920 @default.
- W2039366870 hasRelatedWork W2380360044 @default.
- W2039366870 hasRelatedWork W4292335366 @default.
- W2039366870 hasVolume "57" @default.
- W2039366870 isParatext "false" @default.
- W2039366870 isRetracted "false" @default.
- W2039366870 magId "2039366870" @default.
- W2039366870 workType "article" @default.