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- W2039397629 abstract "Homogenates of rat liver in 0.25 m sucrose were fractionated by differential centrifugation and the intracellular distribution of neuraminidase was studied. The highest relative specific activity was observed in the lysosomal fraction. The supernatant fraction contained the largest percentage of the total enzymic activity present in the whole homogenate. However, when conditions that minimized the manipulation of the particles were utilized, the supernatant fraction had a very low enzyme content. The supernatant fraction from homogenates prepared in isotonic potassium chloride was found to be the most convenient starting material for the determination of total neuraminidase activity in rat organs. Changes of neuraminidase activity were detected in organs of female rats during pregnancy and lactation. Liver and mammary glands showed similar patterns with peaks of activity around 14–16 days after parturition. In brain, peaks were observed about the second week of pregnancy and the first week of lactation." @default.
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- W2039397629 date "1967-01-01" @default.
- W2039397629 modified "2023-10-02" @default.
- W2039397629 title "Mammalian neuraminidase: Intracellular distribution and changes of enzyme activity during lactation" @default.
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- W2039397629 doi "https://doi.org/10.1016/0003-9861(67)90428-6" @default.
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