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- W2039415857 abstract "The gene for the phosphate-starvation-inducible outer membrane protein OprP, of Pseudomonas aeruginosa was introduced into Caulobacter crescentus CB2A on a plasmid vector. As is the case in P. aeruginosa and Escherichia coli the oprP gene was inducible under conditions of limiting phosphate in C. crescentus. However, the maximal medium concentration of phosphate which still permitted induction of OprP was lower in C. crescentus (50 microM) than in P. aeruginosa (200 microM). Induction of OprP was coincident with the process of stalk elongation, known to occur in C. crescentus under phosphate starvation conditions. When induced, OprP was localized to the cell envelope and became a major membrane protein, indicating that the Pseudomonas promoter was efficiently recognized in C. crescentus and that the gene product was targeted to the appropriate region of the cell. Our data provide support for the hypothesis that the mechanism for regulation of phosphate-starvation-inducible genes is highly conserved amongst the eubacteria." @default.
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- W2039415857 date "1991-01-01" @default.
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- W2039415857 title "Expression in<i>Caulobacter crescentus</i>of the phosphate-starvation-inducible porin OprP of<i>Pseudomonas aeruginosa</i>" @default.
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- W2039415857 doi "https://doi.org/10.1111/j.1574-6968.1991.tb04351.x" @default.
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