Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039484489> ?p ?o ?g. }
- W2039484489 endingPage "816" @default.
- W2039484489 startingPage "797" @default.
- W2039484489 abstract "Neuromedin U is a newly described regulatory peptide, found by radioimmunoassay in significant concentrations in both the brain and gut of the rat. The aim of the present study was to localize this peptide immunoreactivity to discrete structures of the gut and brain and to map its distribution using immunocytochemistry. In the gut, neuromedin U was confined to nerve fibres mainly in the myenteric and submucous plexuses and the mucosa of all areas except stomach. Immunoreactive ganglion cells were seen in both ganglionated plexuses and their number did not increase following colchicine administration. This observation and the finding that the population of neuromedin U-immunoreactive nerves in the ileum was not affected by complete extrinsic denervation indicated that the nerves are mostly intrinsic in origin. Colocalization studies revealed neuromedin U and calcitonin gene-related peptide were present in the same myenteric and submucosal ganglion cells. Transection experiments showed that, like calcitonin gene-related peptide-immunoreactive nerves, fibres containing neuromedin U project for very short distances in both an oral and anal direction. At the electron microscopic level, neuromedin U immunoreactivity, demonstrated using the immunogold technique, was localized to large granular vesicles. In the central nervous system, neuromedin U immunoreactivity was localized to fibres which were widespread throughout the brain, except in the cerebellum. The presence of neuromedin U-immunoreactive cell bodies was restricted to the rostrocaudal part of the arcuate nucleus. Colocalization studies showed that a proportion of the neuromedin U-immunoreactive cell bodies in the arcuate nucleus also contained pro-opiomelanocortin. Neuromedin U-immunoreactive fibres were first detected in the rat intestinal mucosa at day 1 after birth. In the brain, the arcuate nucleus showed neuromedin U-immunoreactive neuronal cell bodies at E16 but not at E14. In conclusion, neuromedin U is a new member of the group of molecules known as brain-gut peptides. Since this manuscript was accepted for publication a study describing the immunocytochemical localization of neuromedin U-like immunoreactivity in the rat brain has appeared in the literature [Honzawa et al. (1987) Neuroscience23, 1103–1122]. The reported distribution in the latter paper, however, was at variance with our findings. We have therefore further investigated the distribution of the neuromedin U-like immunoreactivity using a range of antisera from rabbits which have been immunized subsequent to our original work. Two animals produced antisera which stained cell bodies in the cortex, in accord with the report by Honzawa and co-workers. However, in our hands the staining obtained was capricious (seen in five out of 10 rat brains) and could not be abolished by preabsorption of the antiserum with synthetic neuromedin U (10 nmol/ml diluted antiserum). In addition this localization pattern is at variance with data obtained from radioimmunoassay of extracts of cortex. In comparison to other regions of the central nervous system levels of neuromedin U-like-immunoreactive material were the lowest recorded in the cortex by our own group6 and others [Augood et al. (1987) Soc. Neurosci. Abstr.13, 1385]." @default.
- W2039484489 created "2016-06-24" @default.
- W2039484489 creator A5002313687 @default.
- W2039484489 creator A5010084680 @default.
- W2039484489 creator A5011196462 @default.
- W2039484489 creator A5019231390 @default.
- W2039484489 creator A5036042450 @default.
- W2039484489 creator A5059320414 @default.
- W2039484489 creator A5066349102 @default.
- W2039484489 creator A5072872290 @default.
- W2039484489 creator A5076020658 @default.
- W2039484489 creator A5090585484 @default.
- W2039484489 date "1988-06-01" @default.
- W2039484489 modified "2023-10-01" @default.
- W2039484489 title "Occurrence and developmental pattern of neuromedin U-immunoreactive nerves in the gastrointestinal tract and brain of the rat" @default.
- W2039484489 cites W1964373371 @default.
- W2039484489 cites W1972525015 @default.
- W2039484489 cites W1978162138 @default.
- W2039484489 cites W1985487450 @default.
- W2039484489 cites W1985820234 @default.
- W2039484489 cites W1987402546 @default.
- W2039484489 cites W1993620584 @default.
- W2039484489 cites W1998725386 @default.
- W2039484489 cites W2002240256 @default.
- W2039484489 cites W2006387884 @default.
- W2039484489 cites W2009935529 @default.
- W2039484489 cites W2019558153 @default.
- W2039484489 cites W2049829718 @default.
- W2039484489 cites W2060362267 @default.
- W2039484489 cites W2071230078 @default.
- W2039484489 cites W2083421531 @default.
- W2039484489 cites W2089154610 @default.
- W2039484489 cites W2090162012 @default.
- W2039484489 cites W2100720542 @default.
- W2039484489 cites W2108641121 @default.
- W2039484489 cites W2154463038 @default.
- W2039484489 cites W4252714631 @default.
- W2039484489 cites W4300926624 @default.
- W2039484489 doi "https://doi.org/10.1016/0306-4522(88)90037-1" @default.
- W2039484489 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3405430" @default.
- W2039484489 hasPublicationYear "1988" @default.
- W2039484489 type Work @default.
- W2039484489 sameAs 2039484489 @default.
- W2039484489 citedByCount "114" @default.
- W2039484489 countsByYear W20394844892012 @default.
- W2039484489 countsByYear W20394844892013 @default.
- W2039484489 countsByYear W20394844892014 @default.
- W2039484489 countsByYear W20394844892015 @default.
- W2039484489 countsByYear W20394844892016 @default.
- W2039484489 countsByYear W20394844892017 @default.
- W2039484489 countsByYear W20394844892018 @default.
- W2039484489 countsByYear W20394844892019 @default.
- W2039484489 countsByYear W20394844892020 @default.
- W2039484489 countsByYear W20394844892021 @default.
- W2039484489 countsByYear W20394844892022 @default.
- W2039484489 countsByYear W20394844892023 @default.
- W2039484489 crossrefType "journal-article" @default.
- W2039484489 hasAuthorship W2039484489A5002313687 @default.
- W2039484489 hasAuthorship W2039484489A5010084680 @default.
- W2039484489 hasAuthorship W2039484489A5011196462 @default.
- W2039484489 hasAuthorship W2039484489A5019231390 @default.
- W2039484489 hasAuthorship W2039484489A5036042450 @default.
- W2039484489 hasAuthorship W2039484489A5059320414 @default.
- W2039484489 hasAuthorship W2039484489A5066349102 @default.
- W2039484489 hasAuthorship W2039484489A5072872290 @default.
- W2039484489 hasAuthorship W2039484489A5076020658 @default.
- W2039484489 hasAuthorship W2039484489A5090585484 @default.
- W2039484489 hasConcept C105702510 @default.
- W2039484489 hasConcept C118303440 @default.
- W2039484489 hasConcept C126322002 @default.
- W2039484489 hasConcept C134018914 @default.
- W2039484489 hasConcept C163170386 @default.
- W2039484489 hasConcept C170493617 @default.
- W2039484489 hasConcept C2781334511 @default.
- W2039484489 hasConcept C2908647359 @default.
- W2039484489 hasConcept C35866371 @default.
- W2039484489 hasConcept C40209533 @default.
- W2039484489 hasConcept C71924100 @default.
- W2039484489 hasConcept C86803240 @default.
- W2039484489 hasConcept C95444343 @default.
- W2039484489 hasConcept C99454951 @default.
- W2039484489 hasConceptScore W2039484489C105702510 @default.
- W2039484489 hasConceptScore W2039484489C118303440 @default.
- W2039484489 hasConceptScore W2039484489C126322002 @default.
- W2039484489 hasConceptScore W2039484489C134018914 @default.
- W2039484489 hasConceptScore W2039484489C163170386 @default.
- W2039484489 hasConceptScore W2039484489C170493617 @default.
- W2039484489 hasConceptScore W2039484489C2781334511 @default.
- W2039484489 hasConceptScore W2039484489C2908647359 @default.
- W2039484489 hasConceptScore W2039484489C35866371 @default.
- W2039484489 hasConceptScore W2039484489C40209533 @default.
- W2039484489 hasConceptScore W2039484489C71924100 @default.
- W2039484489 hasConceptScore W2039484489C86803240 @default.
- W2039484489 hasConceptScore W2039484489C95444343 @default.
- W2039484489 hasConceptScore W2039484489C99454951 @default.
- W2039484489 hasIssue "3" @default.
- W2039484489 hasLocation W20394844891 @default.
- W2039484489 hasLocation W20394844892 @default.