Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039652517> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W2039652517 endingPage "967" @default.
- W2039652517 startingPage "962" @default.
- W2039652517 abstract "The characteristics of Ca(2+) transport into endoplasmic reticulum vesicles isolated from roots of Lepidium sativum L. cv Krause have been investigated. The concentration of free Ca(2+) and ATP needed for half-maximal activity were 2.5 and 73 micromolar, respectively, and the enzyme obeyed Michaelis-Menten-like kinetics. The pH maximum occurred at 7.5 and the activity was greatly reduced at either pH 7.0 or 8.0.The Ca(2+)-dependent modulation protein, calmodulin, was tested for its effect on Ca(2+) transport into endoplasmic reticulum vesicles. Although the phenothiazine inhibitors chlorpromazine, fluphenazine, and trifluoperazine all inhibited Ca(2+) transport activity with a half-maximal effect at approximately 35 micromolar, authentic bovine brain calmodulin did not alter the activity at concentrations of 0.5 to 8 micrograms per milliliter. Calmodulin also showed no influence on the time-dependent accumulation of Ca(2+) into vesicles. The membranes did not contain endogenously bound calmodulin since washing with (ethylenebis[oxyethylenenitrile])tetraacetic acid or fluphenazine, treatments which disrupt calmodulin binding, did not alter Ca(2+) transport activity. The inhibition of Ca(2+) transport by phenothiazine drugs was likely related to their nonspecific interaction with the membrane. Thus, there was no indication that calmodulin regulated Ca(2+) uptake into root endoplasmic reticulum." @default.
- W2039652517 created "2016-06-24" @default.
- W2039652517 creator A5038982382 @default.
- W2039652517 date "1984-12-01" @default.
- W2039652517 modified "2023-09-27" @default.
- W2039652517 title "Characterization of Ca<sup>2+</sup> Transport in Purified Endoplasmic Reticulum Membrane Vesicles from <i>Lepidium sativum</i> L. Roots" @default.
- W2039652517 cites W1989014446 @default.
- W2039652517 cites W2011895002 @default.
- W2039652517 cites W2128635872 @default.
- W2039652517 cites W2322464912 @default.
- W2039652517 doi "https://doi.org/10.1104/pp.76.4.962" @default.
- W2039652517 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1064416" @default.
- W2039652517 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16663981" @default.
- W2039652517 hasPublicationYear "1984" @default.
- W2039652517 type Work @default.
- W2039652517 sameAs 2039652517 @default.
- W2039652517 citedByCount "62" @default.
- W2039652517 countsByYear W20396525172018 @default.
- W2039652517 countsByYear W20396525172019 @default.
- W2039652517 crossrefType "journal-article" @default.
- W2039652517 hasAuthorship W2039652517A5038982382 @default.
- W2039652517 hasConcept C130316041 @default.
- W2039652517 hasConcept C158617107 @default.
- W2039652517 hasConcept C181199279 @default.
- W2039652517 hasConcept C185592680 @default.
- W2039652517 hasConcept C2775891158 @default.
- W2039652517 hasConcept C29688787 @default.
- W2039652517 hasConcept C41625074 @default.
- W2039652517 hasConcept C55493867 @default.
- W2039652517 hasConceptScore W2039652517C130316041 @default.
- W2039652517 hasConceptScore W2039652517C158617107 @default.
- W2039652517 hasConceptScore W2039652517C181199279 @default.
- W2039652517 hasConceptScore W2039652517C185592680 @default.
- W2039652517 hasConceptScore W2039652517C2775891158 @default.
- W2039652517 hasConceptScore W2039652517C29688787 @default.
- W2039652517 hasConceptScore W2039652517C41625074 @default.
- W2039652517 hasConceptScore W2039652517C55493867 @default.
- W2039652517 hasIssue "4" @default.
- W2039652517 hasLocation W20396525171 @default.
- W2039652517 hasLocation W20396525172 @default.
- W2039652517 hasOpenAccess W2039652517 @default.
- W2039652517 hasPrimaryLocation W20396525171 @default.
- W2039652517 hasRelatedWork W1490746554 @default.
- W2039652517 hasRelatedWork W18150812 @default.
- W2039652517 hasRelatedWork W2023924381 @default.
- W2039652517 hasRelatedWork W2039652517 @default.
- W2039652517 hasRelatedWork W2041478537 @default.
- W2039652517 hasRelatedWork W2060940672 @default.
- W2039652517 hasRelatedWork W2085139421 @default.
- W2039652517 hasRelatedWork W2087185671 @default.
- W2039652517 hasRelatedWork W2100044473 @default.
- W2039652517 hasRelatedWork W2396072780 @default.
- W2039652517 hasVolume "76" @default.
- W2039652517 isParatext "false" @default.
- W2039652517 isRetracted "false" @default.
- W2039652517 magId "2039652517" @default.
- W2039652517 workType "article" @default.