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• W2039654089 endingPage "494" @default.
• W2039654089 startingPage "486" @default.
• W2039654089 abstract "The 5S rRNA gene of the soybean Glycine max (L.) Merr. has been cloned on a 556-bp fragment of DNA and sequenced. This fragment contains two copies of the soybean 5S rDNA sequence, one intact and one truncated, separated by noncoding DNA. We have used this clone to investigate the organization of the 5S genes within the soybean genome and the extent of their methylation. Our results demonstrate that soybean 5S genes are clustered, organized into tandem repeats of 330 bp, and extensively methylated. Hybridization of the 5S sequence to Southern transfers of soybean DNA digested with BamHI reveals a striking ladderlike pattern. Hybridization of the soybean 5S sequence to a wide variety of plant DNAs results in similar patterns, suggesting that the 5S rDNA sequence, gene organization, and methylation pattern are conserved in many higher plants.Key words: 5S rDNA, sequence, methylation, soybean, repeat conservation." @default.
• W2039654089 created "2016-06-24" @default.
• W2039654089 creator A5007199263 @default.
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• W2039654089 date "1990-08-01" @default.
• W2039654089 modified "2023-09-27" @default.
• W2039654089 title "Organization of the 5S rRNA genes in the soybean <i>Glycine max</i> (L.) Merrill and conservation of the 5S rDNA repeat structure in higher plants" @default.
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• W2039654089 doi "https://doi.org/10.1139/g90-072" @default.
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