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• W2039674297 startingPage "1460" @default.
• W2039674297 abstract "Bacterial plasmids may often reach a copy number larger than 1000 per cell, corresponding to a total amount of DNA that may exceed the amount of DNA within the bacterial chromosome. This observation highlights the problem of cellular accommodation of large amounts of closed-circular nucleic acids, whose interwound conformation offers negligible DNA compaction. As determined by x-ray scattering experiments conducted on intact bacteria, supercoiled plasmids segregate within the cells into dense clusters characterized by a long-range order. In vitro studies performed at physiological DNA concentrations indicated that interwound DNA spontaneously forms liquid crystalline phases whose macroscopic structural properties are determined by the features of the molecular supercoiling. Because these features respond to cellular factors, DNA supercoiling may provide a sensitive regulatory link between cellular parameters and the packaging modes of interwound DNA in vivo." @default.
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• W2039674297 date "1994-06-03" @default.
• W2039674297 modified "2023-10-18" @default.
• W2039674297 title "Liquid-Crystalline Mesophases of Plasmid DNA in Bacteria" @default.
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• W2039674297 doi "https://doi.org/10.1126/science.8197460" @default.
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