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• W2039679742 abstract "ABSTRACT The oxidative cleavage of heme to release iron is a mechanism by which some bacterial pathogens can utilize heme as an iron source. The pigA gene of Pseudomonas aeruginosa is shown to encode a heme oxygenase protein, which was identified in the genome sequence by its significant homology (37%) with HemO of Neisseria meningitidis . When the gene encoding the neisserial heme oxygenase, hemO , was replaced with pigA , we demonstrated that pigA could functionally replace hemO and allow for heme utilization by neisseriae. Furthermore, when pigA was disrupted by cassette mutagenesis in P. aeruginosa , heme utilization was defective in iron-poor media supplemented with heme. This defect could be restored both by the addition of exogenous FeSO 4 , indicating that the mutant did not have a defect in iron metabolism, and by in trans complementation with pigA from a plasmid with an inducible promoter. The PigA protein was purified by ion-exchange chromotography. The UV-visible spectrum of PigA reconstituted with heme showed characteristics previously reported for other bacterial and mammalian heme oxygenases. The heme-PigA complex could be converted to ferric biliverdin in the presence of ascorbate, demonstrating the need for an exogenous reductant. Acidification and high-performance liquid chromatography analysis of the ascorbate reduction products identified a major product of biliverdin IX-β. This differs from the previously characterized heme oxygenases in which biliverdin IX-α is the typical product. We conclude that PigA is a heme oxygenase and may represent a class of these enzymes with novel regiospecificity." @default.
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• W2039679742 date "2001-11-01" @default.
• W2039679742 modified "2023-10-13" @default.
• W2039679742 title "Homologues of Neisserial Heme Oxygenase in Gram-Negative Bacteria: Degradation of Heme by the Product of the <i>pigA</i> Gene of <i>Pseudomonas aeruginosa</i>" @default.
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• W2039679742 doi "https://doi.org/10.1128/jb.183.21.6394-6403.2001" @default.
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