FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039708183> ?p ?o ?g. }

• W2039708183 endingPage "3191" @default.
• W2039708183 startingPage "3181" @default.
• W2039708183 abstract "To determine whether oxygen-dependent activation patterns of hypoxia-inducible factor 1alpha (HIF-1alpha) observed in vascularized tissues are conserved within avascular and hypoxic articular cartilage and whether HIF-1alpha affects cartilage matrix synthesis.Explants of bovine articular cartilage and primary chondrocytes were exposed to normoxia (21% O2), hypoxia (2% O2), and simulated hypoxia (21% O2 plus CoCl2). Western blot and immunofluorescence analyses of HIF-1alpha were performed to determine HIF-1alpha activation patterns. To simulate cartilage loss from disease or injury, the top layers of cartilage were removed from osteochondral explants, and the residual cartilage was assessed for HIF-1alpha immunolocalization and proteoglycan synthesis.We demonstrated continuous nuclear translocation of HIF-1alpha in deeper layers of intact articular cartilage. HIF-1alpha was not completely degraded in chondrocytes exposed to normoxia, but rather, colocalized to the Golgi complex, a finding not previously reported for any cell type. Following alteration of the oxygen gradient by removal of the top layers of cartilage, predominantly perinuclear HIF-1alpha was found in the deeper layers. Restoration of intranuclear HIF-1alpha to these areas was achieved by hypoxia and simulated hypoxia. Under conditions in which HIF-1alpha was inactivated, matrix synthetic activity was altered (P < 0.0001) compared with control cartilage.These findings demonstrate that hypoxia-dependent activation of HIF-1alpha is highly conserved and that changes in oxygen tensions following cartilage loss from injury or disease alter cartilage metabolism in part by changing HIF-1alpha activity. The discovery of tonic activation of HIF-1alpha within intact articular cartilage underscores its potential importance to cartilage homeostasis." @default.
• W2039708183 created "2016-06-24" @default.
• W2039708183 creator A5009232412 @default.
• W2039708183 creator A5027623537 @default.
• W2039708183 creator A5040504105 @default.
• W2039708183 date "2005-09-30" @default.
• W2039708183 modified "2023-10-12" @default.
• W2039708183 title "Tonic activation of hypoxia-inducible factor 1α in avascular articular cartilage and implications for metabolic homeostasis" @default.
• W2039708183 cites W1606390515 @default.
• W2039708183 cites W1642882929 @default.
• W2039708183 cites W1899997617 @default.
• W2039708183 cites W1937401270 @default.
• W2039708183 cites W1961133493 @default.
• W2039708183 cites W1970850706 @default.
• W2039708183 cites W1977126721 @default.
• W2039708183 cites W1982534962 @default.
• W2039708183 cites W1984348097 @default.
• W2039708183 cites W1993315780 @default.
• W2039708183 cites W2008246498 @default.
• W2039708183 cites W2009918697 @default.
• W2039708183 cites W2011940076 @default.
• W2039708183 cites W2013532660 @default.
• W2039708183 cites W2014255075 @default.
• W2039708183 cites W2016496152 @default.
• W2039708183 cites W2022369905 @default.
• W2039708183 cites W2022750306 @default.
• W2039708183 cites W2024206845 @default.
• W2039708183 cites W2040031153 @default.
• W2039708183 cites W2040468706 @default.
• W2039708183 cites W2055174629 @default.
• W2039708183 cites W2060871317 @default.
• W2039708183 cites W2069273167 @default.
• W2039708183 cites W2072839085 @default.
• W2039708183 cites W2075696414 @default.
• W2039708183 cites W2079903643 @default.
• W2039708183 cites W2084843851 @default.
• W2039708183 cites W2086236912 @default.
• W2039708183 cites W2089367768 @default.
• W2039708183 cites W2091415443 @default.
• W2039708183 cites W2099397203 @default.
• W2039708183 cites W2105235096 @default.
• W2039708183 cites W2105389636 @default.
• W2039708183 cites W2117236885 @default.
• W2039708183 cites W2118172082 @default.
• W2039708183 cites W2120090919 @default.
• W2039708183 cites W2125531516 @default.
• W2039708183 cites W2125724189 @default.
• W2039708183 cites W2127205902 @default.
• W2039708183 cites W2134666212 @default.
• W2039708183 cites W2135836641 @default.
• W2039708183 cites W2138033100 @default.
• W2039708183 cites W2149463866 @default.
• W2039708183 cites W2150461606 @default.
• W2039708183 cites W2166138837 @default.
• W2039708183 cites W2261106200 @default.
• W2039708183 cites W2399049247 @default.
• W2039708183 cites W3211379443 @default.
• W2039708183 cites W49974486 @default.
• W2039708183 cites W53133536 @default.
• W2039708183 doi "https://doi.org/10.1002/art.21346" @default.
• W2039708183 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16200622" @default.
• W2039708183 hasPublicationYear "2005" @default.
• W2039708183 type Work @default.
• W2039708183 sameAs 2039708183 @default.
• W2039708183 citedByCount "38" @default.
• W2039708183 countsByYear W20397081832012 @default.
• W2039708183 countsByYear W20397081832013 @default.
• W2039708183 countsByYear W20397081832014 @default.
• W2039708183 countsByYear W20397081832015 @default.
• W2039708183 countsByYear W20397081832017 @default.
• W2039708183 countsByYear W20397081832018 @default.
• W2039708183 countsByYear W20397081832019 @default.
• W2039708183 countsByYear W20397081832020 @default.
• W2039708183 countsByYear W20397081832021 @default.
• W2039708183 countsByYear W20397081832022 @default.
• W2039708183 crossrefType "journal-article" @default.
• W2039708183 hasAuthorship W2039708183A5009232412 @default.
• W2039708183 hasAuthorship W2039708183A5027623537 @default.
• W2039708183 hasAuthorship W2039708183A5040504105 @default.
• W2039708183 hasBestOaLocation W20397081831 @default.
• W2039708183 hasConcept C104317684 @default.
• W2039708183 hasConcept C105702510 @default.
• W2039708183 hasConcept C134018914 @default.
• W2039708183 hasConcept C142724271 @default.
• W2039708183 hasConcept C160450060 @default.
• W2039708183 hasConcept C178790620 @default.
• W2039708183 hasConcept C185592680 @default.
• W2039708183 hasConcept C204787440 @default.
• W2039708183 hasConcept C2776164576 @default.
• W2039708183 hasConcept C2779335624 @default.
• W2039708183 hasConcept C2780550940 @default.
• W2039708183 hasConcept C540031477 @default.
• W2039708183 hasConcept C55493867 @default.
• W2039708183 hasConcept C71924100 @default.
• W2039708183 hasConcept C7836513 @default.
• W2039708183 hasConcept C86803240 @default.
• W2039708183 hasConcept C95444343 @default.
• W2039708183 hasConceptScore W2039708183C104317684 @default.

• next