Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039763889> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2039763889 endingPage "1377" @default.
- W2039763889 startingPage "1362" @default.
- W2039763889 abstract "Previously, assays for sister chromatid segregation patterns relied on incorporation of 5-bromo-2'-deoxyuridine (BrdU) and indirect methods to infer segregation patterns after two cell divisions. In this study, we describe a method to differentially label sister chromatids of mouse cells and to directly assay sister chromatid segregation patterns after one cell division in vitro and in vivo by adaptation of the well-established CO-FISH technique. BrdU is incorporated into newly formed DNA strands, which are then subjected to photolysis and exonuclease digestion to create single-stranded sister chromatids containing parental template DNA only. Such single-stranded sister chromatids are differentially labeled using unidirectional probes to major satellite sequences coupled to fluorescent markers. Differentially labeled sister chromatids in postmitotic cells are visualized using fluorescence microscopy, and sister chromatid segregation patterns can be directly assayed after one cell division. This procedure requires 4 d for in vivo mouse tissues and 2 d for in vitro-cultured cells." @default.
- W2039763889 created "2016-06-24" @default.
- W2039763889 creator A5009491997 @default.
- W2039763889 creator A5023799679 @default.
- W2039763889 creator A5056104532 @default.
- W2039763889 creator A5066428428 @default.
- W2039763889 date "2010-07-01" @default.
- W2039763889 modified "2023-09-24" @default.
- W2039763889 title "Chromosome orientation fluorescence in situ hybridization to study sister chromatid segregation in vivo" @default.
- W2039763889 cites W1969112301 @default.
- W2039763889 cites W1973188629 @default.
- W2039763889 cites W1987510367 @default.
- W2039763889 cites W1997857277 @default.
- W2039763889 cites W1998672600 @default.
- W2039763889 cites W2038256059 @default.
- W2039763889 cites W2039806656 @default.
- W2039763889 cites W2063075008 @default.
- W2039763889 cites W2064626140 @default.
- W2039763889 cites W2074371275 @default.
- W2039763889 cites W2081193579 @default.
- W2039763889 cites W2089108952 @default.
- W2039763889 cites W2091757969 @default.
- W2039763889 cites W2096157760 @default.
- W2039763889 cites W2106897784 @default.
- W2039763889 cites W2117414780 @default.
- W2039763889 cites W2119440700 @default.
- W2039763889 cites W2136001703 @default.
- W2039763889 cites W2138041459 @default.
- W2039763889 cites W2323640443 @default.
- W2039763889 cites W2343724130 @default.
- W2039763889 doi "https://doi.org/10.1038/nprot.2010.102" @default.
- W2039763889 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3771506" @default.
- W2039763889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20595964" @default.
- W2039763889 hasPublicationYear "2010" @default.
- W2039763889 type Work @default.
- W2039763889 sameAs 2039763889 @default.
- W2039763889 citedByCount "12" @default.
- W2039763889 countsByYear W20397638892012 @default.
- W2039763889 countsByYear W20397638892013 @default.
- W2039763889 countsByYear W20397638892014 @default.
- W2039763889 countsByYear W20397638892015 @default.
- W2039763889 countsByYear W20397638892016 @default.
- W2039763889 countsByYear W20397638892017 @default.
- W2039763889 countsByYear W20397638892021 @default.
- W2039763889 crossrefType "journal-article" @default.
- W2039763889 hasAuthorship W2039763889A5009491997 @default.
- W2039763889 hasAuthorship W2039763889A5023799679 @default.
- W2039763889 hasAuthorship W2039763889A5056104532 @default.
- W2039763889 hasAuthorship W2039763889A5066428428 @default.
- W2039763889 hasBestOaLocation W20397638892 @default.
- W2039763889 hasConcept C104317684 @default.
- W2039763889 hasConcept C1491633281 @default.
- W2039763889 hasConcept C153911025 @default.
- W2039763889 hasConcept C2777542201 @default.
- W2039763889 hasConcept C2779071816 @default.
- W2039763889 hasConcept C30481170 @default.
- W2039763889 hasConcept C54355233 @default.
- W2039763889 hasConcept C552990157 @default.
- W2039763889 hasConcept C73397532 @default.
- W2039763889 hasConcept C8502773 @default.
- W2039763889 hasConcept C85813293 @default.
- W2039763889 hasConcept C86803240 @default.
- W2039763889 hasConcept C95444343 @default.
- W2039763889 hasConceptScore W2039763889C104317684 @default.
- W2039763889 hasConceptScore W2039763889C1491633281 @default.
- W2039763889 hasConceptScore W2039763889C153911025 @default.
- W2039763889 hasConceptScore W2039763889C2777542201 @default.
- W2039763889 hasConceptScore W2039763889C2779071816 @default.
- W2039763889 hasConceptScore W2039763889C30481170 @default.
- W2039763889 hasConceptScore W2039763889C54355233 @default.
- W2039763889 hasConceptScore W2039763889C552990157 @default.
- W2039763889 hasConceptScore W2039763889C73397532 @default.
- W2039763889 hasConceptScore W2039763889C8502773 @default.
- W2039763889 hasConceptScore W2039763889C85813293 @default.
- W2039763889 hasConceptScore W2039763889C86803240 @default.
- W2039763889 hasConceptScore W2039763889C95444343 @default.
- W2039763889 hasIssue "7" @default.
- W2039763889 hasLocation W20397638891 @default.
- W2039763889 hasLocation W20397638892 @default.
- W2039763889 hasLocation W20397638893 @default.
- W2039763889 hasOpenAccess W2039763889 @default.
- W2039763889 hasPrimaryLocation W20397638891 @default.
- W2039763889 hasRelatedWork W1191407461 @default.
- W2039763889 hasRelatedWork W1971118090 @default.
- W2039763889 hasRelatedWork W1989319777 @default.
- W2039763889 hasRelatedWork W2003687085 @default.
- W2039763889 hasRelatedWork W2008823287 @default.
- W2039763889 hasRelatedWork W2026413191 @default.
- W2039763889 hasRelatedWork W2045336243 @default.
- W2039763889 hasRelatedWork W2399068922 @default.
- W2039763889 hasRelatedWork W2486760758 @default.
- W2039763889 hasRelatedWork W2464404580 @default.
- W2039763889 hasVolume "5" @default.
- W2039763889 isParatext "false" @default.
- W2039763889 isRetracted "false" @default.
- W2039763889 magId "2039763889" @default.
- W2039763889 workType "article" @default.