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- W2039772643 abstract "Cholera toxin B (CTB) is often envisaged and used as an immune stimulating agent in protocols for mucosal immunization. However, the nature of the CTB used (natural vs recombinant) is frequently not taken in consideration. This is important since the usage of natural CTB in mucosal immunization regimen and the mucosal response resulting from such an immunization can be effected by the presence of the CTA subunit in commercial CTB preparations. To clarify this, we have compared natural vs recombinant CTB in an intranasal (i.n.) mucosal immunization procedure using ovalbumin (OVA) as antigen. The results show that recombinant CTB induces similar immune responses like natural CTB. Furthermore, our experiments show that covalent coupling of OVA to CTB is not required for the induction of OVA specific mucosal and systemic immune responses upon i.n. immunization" @default.
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- W2039772643 date "1997-07-01" @default.
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- W2039772643 title "A comparison of natural and recombinant cholera toxin B subunit as stimulatory factors in intranasal immunization" @default.
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- W2039772643 doi "https://doi.org/10.1016/s0264-410x(97)00007-8" @default.
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