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• W2039776816 abstract "θ-Toxin (perfringolysin O) of Clostridium perfringens binds to membrane cholesterol with high (Kd ≈ 10−9M) and low (Kd ≈ 10−7M) affinities and causes membrane lysis of intact cells and liposomes. In order to understand the lytic process at the molecular level, the lysis of liposomes was investigated in comparison with that of intact cells. The toxin dose required to cause 50% lysis (RD50) of phosphatidylcholine/phosphatidylglycerol (82:18, mol/mol) liposomes containing 36–40 mol% cholesterol was 300–1400-times higher than the RD50 value for sheep or human erythrocytes when samples with the same cholesterol concentration were compared. However, the average number of toxin molecules bound per liposome vesicle at 50% lysis was estimated as 10–18 from the RD50 values, close to the number on erythrocytes at 50% lysis, suggesting that the number of toxin molecules adsorbed per vesicle is important for lysis. As to the toxin dose required for membrane lysis, no significant difference was observed between liposomes containing both high- and low-affinity toxin-binding sites and those containing only low-affinity sites, suggesting that θ-toxin molecules bound to low-affinity sites can assemble and cause membrane lysis as well as those bound to high-affinity sites. θ-Toxin assembles on liposomal membranes, as on erythrocytes, in a high-molecular-weight polymeric form as judged from sedimentation patterns in sucrose density-gradient centrifugation. The high-molecular-weight polymers were detected only under conditions where cell or liposome lysis occurred. At low toxin doses, slower sedimenting toxin oligomers and monomers were predominant on liposomal membranes. These results indicate that toxin assembly on membranes is essential for liposome lysis as it is for cell lysis and that assembly occurs on membranes without membrane proteins." @default.
• W2039776816 created "2016-06-24" @default.
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• W2039776816 date "1993-11-01" @default.
• W2039776816 modified "2023-10-16" @default.
• W2039776816 title "Membrane disorganization induced by perfringolysin O (theta-toxin) of Clostridium perfringens — effect of toxin binding and self-assembly on liposomes" @default.
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• W2039776816 doi "https://doi.org/10.1016/0005-2736(93)90279-9" @default.
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