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• W2039779035 endingPage "1102" @default.
• W2039779035 startingPage "1089" @default.
• W2039779035 abstract "ABSTRACT The properties and regulation of the pstSCAB -encoded P i uptake system from the alfalfa symbiont Sinorhizobium meliloti are reported. We present evidence that the pstSCAB genes and the regulatory phoUB genes are transcribed from a single promoter that contains two PhoB binding sites and that transcription requires PhoB. S. meliloti strain 1021 (Rm1021) and its derivatives were found to carry a C deletion frameshift mutation in the pstC gene (designated pstC1021 ) that severely impairs activity of the PstSCAB P i transport system. This mutation is absent in RCR2011, the parent of Rm1021. Correction of the pstC1021 mutation in Rm1021 by site-directed mutagenesis revealed that PstSCAB is a P i -specific, high-affinity ( K m , 0.2 μM), high-velocity ( V max , 70 nmol/min/mg protein) transport system. The pstC1021 allele was shown to generate a partial pho regulon constitutive phenotype, in which transcription is activated by PhoB even under P i -excess conditions that render PhoB inactive in a wild-type background. The previously reported symbiotic Fix − phenotype of phoCDET mutants was found to be dependent on the pstC1021 mutation, as Rm1021 phoCDET mutants formed small white nodules on alfalfa that failed to reduce N 2 , whereas phoCDET mutant strains with a corrected pstC allele (RmP110) formed pink nodules on alfalfa that fixed N 2 like the wild type. Alfalfa root nodules formed by the wild-type RCR2011 strain expressed the low-affinity orfA-pit -encoded P i uptake system and neither the pstSCAB genes nor the phoCDET genes. Thus, metabolism of alfalfa nodule bacteroids is not P i limited." @default.
• W2039779035 created "2016-06-24" @default.
• W2039779035 creator A5000015314 @default.
• W2039779035 creator A5033995070 @default.
• W2039779035 creator A5056585256 @default.
• W2039779035 date "2006-02-01" @default.
• W2039779035 modified "2023-10-01" @default.
• W2039779035 title "Regulation and Properties of PstSCAB, a High-Affinity, High-Velocity Phosphate Transport System of <i>Sinorhizobium meliloti</i>" @default.
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• W2039779035 doi "https://doi.org/10.1128/jb.188.3.1089-1102.2006" @default.
• W2039779035 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1347321" @default.
• W2039779035 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16428413" @default.
• W2039779035 hasPublicationYear "2006" @default.
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