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- W2039793350 abstract "Chemical-ionization mass spectrometry (CIMS) using flow reactors is an emerging method for on-line monitoring of trace concentrations of organic compounds in the gas phase. In this study, a flow-reactor CIMS instrument, employing the H(3)O(+) cation as the ionizing reagent, was used to simultaneously monitor several volatile metabolic products as they are released into the headspace during bacterial growth in a bioreactor. Production of acetaldehyde, ethanol, acetone, butanol, acetoin, diacetyl, and isoprene by Bacillus subtilis is reported. Ion signal intensities were related to solution-phase concentrations using empirical calibrations and, in the case of isoprene, were compared with simultaneous gas chromatography measurements. Identification of volatile and semivolatile metabolites is discussed. Flow-reactor CIMS techniques should be useful for bioprocess monitoring applications because of their ability to sensitively and simultaneously monitor many volatile metabolites on-line." @default.
- W2039793350 created "2016-06-24" @default.
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- W2039793350 date "2008-09-05" @default.
- W2039793350 modified "2023-10-14" @default.
- W2039793350 title "Potential of On-Line CIMS for Bioprocess Monitoring" @default.
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- W2039793350 doi "https://doi.org/10.1021/bp025730k" @default.
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