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- W2039807710 abstract "We compared the effects of LPSs from P.gingivalis and P.heparinolytica on the induction of tumor necrosis factor-α (TNF-α) production by murine peritoneal macrophages. The lipopolysaccharide (LPS) preparation from P.gingivalis showed a typical ladder pattern in SDS-PAGE, whereas that from P.heparinolytica formed several stained bands without a ladder pattern. When the macrophages from C3H/HeJ mice were incubated with P.gingivalis LPS, the level of TNF-α released in the culture supernatants was significantly higher than that with P.heparinolytica. All tested reagents except genistein strongly inhibited the production of TNF-α by the macrophages after induction by either LPS. These results suggested the following possibilities, i) the induction level of TNF-α by P.heparinolytica is similar to that of Salmonella minnesota, ii) tyrosine phosphorylation is not the only pathway for the TNF-α production induced by these LPSs, iii) different regulatory mechanisms are involved in TNF-α production in P.gingivalis LPS stimulated and P.heparinolytica LPS stimulated cells." @default.
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- W2039807710 date "2000-01-01" @default.
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- W2039807710 title "Differences in TNF-.ALPHA. Producing Activity from Murine Peritoneal Macrophages Induced by Lipopolysaccharides of Prevotella heparinolytica and Porphyromonas gingivalis." @default.
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