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• W2039813523 abstract "A target RNA/DNA-specific nuclease could be constructed if a specific RNA/DNA binding domain allowing target RNA/DNA recognition was fused to a (deoxy)ribonucleolytic domain allowing target RNA/ DNA cleavage. The design and construction of such a chimeric enzyme could be of value for both basic research involving structure-function relationships and applied research requiring inactivation of harmful RNA/DNA molecules of cellular or pathogenic origin. The feasibility of this designer nuclease approach for inactivating specific RNA/DNA molecules was assessed using human immunodeficiency virus type-1 (HIV-1) RNA as a model. Trans-activator of transcription (Tat) protein is one of the key regulatory proteins encoded by HIV-1. It binds to the trans-activation-responsive (TAR) RNA element located within the 5' non-coding region of HIV-1 RNAs. The TAR RNA binding domain of this protein was fused to the ribonuclease (RNase) H domain of HIV-1 reverse transcriptase (RT). RNase H by itself lacks an RNA binding domain. The chimeric Tat-RNase H protein was shown to specifically recognize and cleave HIV-1 TAR RNA in vitro. Cleavage was abolished by mutations in the Tat binding region within the TAR RNA, indicating that it is specific to HIV-1 TAR RNA." @default.
• W2039813523 created "2016-06-24" @default.
• W2039813523 creator A5026969437 @default.
• W2039813523 creator A5052985547 @default.
• W2039813523 date "1996-05-15" @default.
• W2039813523 modified "2023-10-17" @default.
• W2039813523 title "Fusion with an RNA binding domain to confer target RNA specificity to an RNase: design and engineering of Tat-RNase H that specifically recognizes and cleaves HIV-1 RNA in vitro" @default.
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• W2039813523 doi "https://doi.org/10.1093/nar/24.10.1908" @default.
• W2039813523 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/145861" @default.
• W2039813523 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8657573" @default.
• W2039813523 hasPublicationYear "1996" @default.
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