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- W2039842729 abstract "Cytochemical analysis of Streptomyces coelicolor A3(2) indicated that the aerial growth rodlet mosaic is a polysaccharide. Statistical analysis of frequency distributions of individual rodlet lengths from control and ether-reoriented spore mosaics indicated that the rodlet fibrillar image is the result of individual particulates, rather than evaginations in a continuous sheet of material. A model of the mature spore envelope was developed from freeze-etch-replicated, thin-sectioned, and critical point dried S. coelicolor A3(2) mature spores. The rodlet mosaic was situated between the outer spore wall and an external granular matrix. Mature spore envelope layers from the inner surface to the external surface are plasma membrane, inner spore wall, outer spore wall, rodlet mosaic, an undefined granular matrix, and the sheath. The granular matrix had an uneven thickness and much of the matrix was frequently absent from the interspore spaces of mature spore chains. Streptomyces coelicolor A3(2) mosaic rodlets were isolated by acetic acid refluxing, then ethanol precipitation. Complete acid hydrolysis of rodlets released one sugar which cochromatographed with D-glucosamine-HCl and released acetic acid at 139% of the expected level. Cell-associated rodlet mosaics and isolated mosaic rodlets were hydrolyzed with chitinase. Infrared spectra of isolated rodlets were similar to crab chitin spectra." @default.
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- W2039842729 date "1978-04-01" @default.
- W2039842729 modified "2023-09-26" @default.
- W2039842729 title "Characteristics of Streptomyces coelicolor A3(2) aerial spore rodlet mosaic" @default.
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- W2039842729 doi "https://doi.org/10.1139/m78-066" @default.
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