Matches in SemOpenAlex for { <https://semopenalex.org/work/W2039856732> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2039856732 endingPage "650" @default.
- W2039856732 startingPage "643" @default.
- W2039856732 abstract "Phialocephala fortinii is a dark septate fungal endophyte that colonizes roots of many host species. Its effect on plant growth varies from being pathogenic to beneficial. The basic biology of this species has received little research, and thus the main objectives of this study were to determine cytological features of hyphae, including the nature of the vacuolar system, and whether polyphosphate was present in vacuoles. Both living hyphae and hyphae that had been rapidly frozen and freeze substituted before embedding were studied. A complex system of vacuoles, including a motile tubular vacuolar system, elongated vacuoles, and spherical vacuoles, was demonstrated in living hyphae by the fluorescent probe Oregon Green 488 carboxylic acid diacetate, using laser scanning confocal microscopy. The motile tubular vacuolar system was more prevalent at the hyphal tip than in more distal regions, whereas elongated vacuoles and spherical vacuoles were more abundant distal to the tip. All vacuoles contained polyphosphate as shown by labelling embedded samples with recombinant polyphosphate binding domain of Escherichia coli exopolyphosphatase, containing Xpress tag at the N-terminal end, followed by anti-Xpress antibody and a secondary antibody conjugated either to a fluorescent probe for laser scanning confocal microscopy or colloidal gold for transmission electron microscopy. The polyphosphate was dispersed in vacuoles. This was confirmed by staining embedded samples with 4′,6-diamidino-2-phenylindole and viewing with UV light using epifluorescence microscopy. These cytological methods showed that the tubular vacuolar system had lower concentrations of polyphosphate than the spherical vacuoles. Lipid bodies were present around vacuoles.Key words: Phialocephala, motile tubular vacuoles, vacuoles, polyphosphate, lipid." @default.
- W2039856732 created "2016-06-24" @default.
- W2039856732 creator A5008395353 @default.
- W2039856732 creator A5009904425 @default.
- W2039856732 creator A5060785947 @default.
- W2039856732 creator A5089552993 @default.
- W2039856732 date "2006-07-01" @default.
- W2039856732 modified "2023-09-23" @default.
- W2039856732 title "Vacuolar localization of phosphorus in hyphae of<i>Phialocephala fortinii</i>, a dark septate fungal root endophyte" @default.
- W2039856732 cites W1964100283 @default.
- W2039856732 cites W2000593151 @default.
- W2039856732 cites W2013213357 @default.
- W2039856732 cites W2030646129 @default.
- W2039856732 cites W2038229936 @default.
- W2039856732 cites W2043240048 @default.
- W2039856732 cites W2061385055 @default.
- W2039856732 cites W2061420092 @default.
- W2039856732 cites W2064850868 @default.
- W2039856732 cites W2067176660 @default.
- W2039856732 cites W2071812646 @default.
- W2039856732 cites W2076308228 @default.
- W2039856732 cites W2079288734 @default.
- W2039856732 cites W2109401564 @default.
- W2039856732 cites W2120973540 @default.
- W2039856732 cites W2122268960 @default.
- W2039856732 cites W2124952973 @default.
- W2039856732 cites W2125738978 @default.
- W2039856732 cites W2126102177 @default.
- W2039856732 cites W2137559260 @default.
- W2039856732 cites W2139086009 @default.
- W2039856732 cites W2148604344 @default.
- W2039856732 cites W2154329063 @default.
- W2039856732 cites W2267561920 @default.
- W2039856732 cites W4247271713 @default.
- W2039856732 cites W63522944 @default.
- W2039856732 doi "https://doi.org/10.1139/w06-018" @default.
- W2039856732 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16917520" @default.
- W2039856732 hasPublicationYear "2006" @default.
- W2039856732 type Work @default.
- W2039856732 sameAs 2039856732 @default.
- W2039856732 citedByCount "24" @default.
- W2039856732 countsByYear W20398567322012 @default.
- W2039856732 countsByYear W20398567322013 @default.
- W2039856732 countsByYear W20398567322014 @default.
- W2039856732 countsByYear W20398567322016 @default.
- W2039856732 countsByYear W20398567322017 @default.
- W2039856732 countsByYear W20398567322018 @default.
- W2039856732 countsByYear W20398567322020 @default.
- W2039856732 countsByYear W20398567322021 @default.
- W2039856732 countsByYear W20398567322022 @default.
- W2039856732 crossrefType "journal-article" @default.
- W2039856732 hasAuthorship W2039856732A5008395353 @default.
- W2039856732 hasAuthorship W2039856732A5009904425 @default.
- W2039856732 hasAuthorship W2039856732A5060785947 @default.
- W2039856732 hasAuthorship W2039856732A5089552993 @default.
- W2039856732 hasConcept C102568950 @default.
- W2039856732 hasConcept C12554922 @default.
- W2039856732 hasConcept C190062978 @default.
- W2039856732 hasConcept C2777132085 @default.
- W2039856732 hasConcept C2778038992 @default.
- W2039856732 hasConcept C55493867 @default.
- W2039856732 hasConcept C59822182 @default.
- W2039856732 hasConcept C86803240 @default.
- W2039856732 hasConcept C90080823 @default.
- W2039856732 hasConceptScore W2039856732C102568950 @default.
- W2039856732 hasConceptScore W2039856732C12554922 @default.
- W2039856732 hasConceptScore W2039856732C190062978 @default.
- W2039856732 hasConceptScore W2039856732C2777132085 @default.
- W2039856732 hasConceptScore W2039856732C2778038992 @default.
- W2039856732 hasConceptScore W2039856732C55493867 @default.
- W2039856732 hasConceptScore W2039856732C59822182 @default.
- W2039856732 hasConceptScore W2039856732C86803240 @default.
- W2039856732 hasConceptScore W2039856732C90080823 @default.
- W2039856732 hasIssue "7" @default.
- W2039856732 hasLocation W20398567321 @default.
- W2039856732 hasLocation W20398567322 @default.
- W2039856732 hasOpenAccess W2039856732 @default.
- W2039856732 hasPrimaryLocation W20398567321 @default.
- W2039856732 hasRelatedWork W1546563164 @default.
- W2039856732 hasRelatedWork W2039856732 @default.
- W2039856732 hasRelatedWork W2081086586 @default.
- W2039856732 hasRelatedWork W2089654828 @default.
- W2039856732 hasRelatedWork W2093643488 @default.
- W2039856732 hasRelatedWork W2096592361 @default.
- W2039856732 hasRelatedWork W2124952973 @default.
- W2039856732 hasRelatedWork W2157476961 @default.
- W2039856732 hasRelatedWork W2170258623 @default.
- W2039856732 hasRelatedWork W4247271713 @default.
- W2039856732 hasVolume "52" @default.
- W2039856732 isParatext "false" @default.
- W2039856732 isRetracted "false" @default.
- W2039856732 magId "2039856732" @default.
- W2039856732 workType "article" @default.