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- W2039935701 abstract "From suspension cultures of the embryogenic D. lanata cell strain VII, plants were regenerated via somatic embryos. Embryo formation was triggered by withdrawal of the auxin 2,4-D from the nutrient solution. Embryogenesis and the further development of the embryos to plants proceeded in hormonefree nutrient media. Use of a liquid nutrient medium with maltose as carbon source, sorbitol as an osmotic, and NH4+ and NO3- as nitrogen sources in the ratio 1:11 was most suitable for formation of large numbers of embryos. The addition of ABA at the beginning of embryogenesis increased the rate of morphologically normal embryos and plants. The plants formed in liquid medium were further cultivated on perlite impregnated with hormone-free nutrient solution. After 4 weeks the plants were adapted to the conditions of the greenhouse. They developed normally, but showed large differences in the cardenolide contents." @default.
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- W2039935701 date "1990-12-01" @default.
- W2039935701 modified "2023-09-29" @default.
- W2039935701 title "Regeneration of plants from somatic embryos of Digitalis lanata" @default.
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- W2039935701 doi "https://doi.org/10.1016/s0176-1617(11)80085-4" @default.
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