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- W2040080011 abstract "Abstract. The DNA synthesis time (Ts) of lymphocytes from spleens and lymph nodes of patients with Hodgkin's disease was determined by the double labeling method. 3H-TdR was administered in vivo and removed tissues minced in 14C-TdR in vitro. Lymphocytes from patients with lymphosarcoma and reticulum cell sarcoma were studied in a similar manner. Lymphocytes were divided into A cells, small with non-basophilic cytoplasm, B cells, small with basophilic cytoplasm, C cells, large with non-basophilic cytoplasm, and D cells, large with basophilic cytoplasm. The Ts of splenic lymphocytes, in four samples not containing Reed-Sternberg cells, in hours, was: B 11.3; C, 7.9; D, 8.4; combined, 8.8. the Ts of B lymphocytes was significantly longer than that of C and D lymphocytes. A lymphocytes did not label sufficiently to measure Ts. C and D lymph node lymphocytes and lymphocytes in tissues containing Reed-Sternberg cells had a longer Ts than splenic C and D cells. the former was: B, 12.7; C, 11.7; D, 11.0; combined, 11.8. the latter was: B, 12.2; C, 11.3; D, 11.2; combined, 11.6. the Ts of Reed-Sternberg cells in one specimen of a splenic Hodgkin's tumor was 13.1 hr. Macrophage Ts was 10.7 and 15.1 hr. Lymphosarcoma cell Ts was 14.2 and 14.6 hr. Reticulum cell sarcoma cell Ts was 7.5 and 7.7 hr. The following minimum times were calculated from observation of 3H-TdR only labeled mitotic figures: S to prophase, 71 min; S to metaphase, 75 min; S to telophase, 100 min." @default.
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- W2040080011 date "1971-11-01" @default.
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- W2040080011 title "Human Lymphocyte Proliferation: Dna Synthesis Time" @default.
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- W2040080011 doi "https://doi.org/10.1111/j.1365-2184.1971.tb01566.x" @default.
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