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- W2040095536 abstract "The ATP photoaffinity analogue 8-azidoadenosine 5′-triphosphate (8N3ATP) was used to identify changes which occur in ATP binding proteins in Drosophila salivary glands following heat shock. Photolabeling experiments were done on salivary gland homogenates. Photoincorporation of 8N3ATP was observed in several proteins in both 25°C control and 35°C heat-shocked samples. A 42 kDa protein showed a decrease in the level of photoincorporation observed at saturation with the large analogue following heat shock. A 2 min heat shock is enough to induce the effect. Protection against photolabeling was observed with low concentrations (5 μM) of ATP, while excess GTP did not protect, demonstrating that the nucleotide binding site is specific for ATP. The change is rapid enough to suggest that it is one of the earliest cellular changes in response to heat shock." @default.
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- W2040095536 date "1990-04-01" @default.
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- W2040095536 title "Heat shock induces a decrease in incorporation of 8-azidoadenosine 5′-triphosphate into a 42 kilodalton protein in Drosophila salivary glands" @default.
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- W2040095536 doi "https://doi.org/10.1016/0304-4165(90)90159-t" @default.
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