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- W2040147283 abstract "A procedure has been described for the specific and rapid isolation of rat urinary kallikrein using anion-exchange, affinity and Sephadex chromatography. Trasylol-bound Sepharose selectively adsorbed kallikrein which could be eluted between pH 5.5 and 3.5. The kallikrein was purified 694-fold by the entire procedure and 49 times in the individual step of affinity chromatography. The purification obtained by the Trasylol-Sepharose adsorption was one of the highest of any individual steps reported thus far. Antibodies generated against this kallikrein reacted with rat urine and kidney cortex homogenate, but not with bovine trypsin, dog or human urine. The activity in vitro and in vivo of kallikrein was specifically blocked by the antikallikrein serum." @default.
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- W2040147283 date "1976-07-01" @default.
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- W2040147283 title "Isolation of rat urinary kallikrein and properties of its antibodies" @default.
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- W2040147283 doi "https://doi.org/10.1016/0006-2952(76)90471-8" @default.
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