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- W2040147673 abstract "An automated vision image analysis system to analyze images resulting from nucleic acid hybridizations is presented. Real-time image acquisition from autoradiographs is accomplished with a sensitivity to optical density to the degree that any spot visible by eye can be quantified by digital methods and enhanced by image processing techniques for observation. The system provides a tool for quantification of differences in gene expression, rapid processing of thousands of pairs of genes, storage in a database, search for small differences, large differences, and infrequently occuring genes. Background correction, normalization and pseudocolor "mapping" facilitate comparison and quantification directly from the CRT display. The image pairs, a percent change image and a difference image are presented in a quad display with coordinated spot density and change measurement simultaneously for each of the four images. The system provides a tool for analysis of altered gene expression in response to environmental stress, such as heat-shock and salt water in plant growth; hormone regulation, such as before and after hormone administration in animals; development; and carcinogenesis. The use of this system to study nucleic acid hybridization is contrasted with the study of 2-D protein electrophoresis gels for the determination of changes in gene expression and identification of corresponding proteins of significance." @default.
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- W2040147673 date "1987-06-03" @default.
- W2040147673 modified "2023-09-23" @default.
- W2040147673 title "Object Identification And Measurement From Images With Access To The Database To Select Specific Subpopulations Of Special Interest" @default.
- W2040147673 doi "https://doi.org/10.1117/12.940046" @default.
- W2040147673 hasPublicationYear "1987" @default.
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