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- W2040173685 abstract "A cDNA encoding the rat enkephalinase protein (neutral endopeptidase; EC 3.4.24.11) has been constructed from overlapping > 10 cDNA clones. This cDNA was inserted into an expression plasmid containing the cytomegalovirus enhancer and promoter. When transfected with this plasmid, Cos 7 cells transiently expressed the enkephalinase protein in a membrane-bound state. Recombinant enkephalinase recovered in solubilized extracts from transfected Cos 7 cells was enzymatically active and displayed properties similar to those of the native enzyme with respect to sensitivity to classical enkephalinase inhibitors." @default.
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- W2040173685 date "1989-03-01" @default.
- W2040173685 modified "2023-09-27" @default.
- W2040173685 title "Expression of enzymatically active enkephalinase (neutral endopeptidase) in mammalian cells" @default.
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- W2040173685 doi "https://doi.org/10.1002/jcb.240390307" @default.
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