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- W2040193953 abstract "A gene-specific transcription factor, called USF, has been partially purified from HeLa cell nuclear extracts. Addition of USF results in a 10 to 20 fold increase in transcription from the adenovirus major late promoter in an in vitro system reconstituted with transcription factors TFIIB, TFIID, TFIIE, and RNA polymerase II. Binding of USF to the promoter inhibits DNAase I cleavages over a 20 base pair region just upstream of the -45 to +35 region shown previously to interact with TFIID. More discriminating footprint analyses using methidiumpropyl-EDTA-Fe(II) as the cleaving agent indicate that USF interacts primarily with the small palindromic DNA sequence GGCCACGTGACC located between positions -63 and -52 of the major late promoter, while TFIID interacts primarily with a 10 base pair DNA region centered on the consensus TATA sequence. Dissociation rate measurements indicate a cooperative interaction between USF and TFIID when simultaneously bound to the promoter DNA." @default.
- W2040193953 created "2016-06-24" @default.
- W2040193953 creator A5007755251 @default.
- W2040193953 creator A5085248591 @default.
- W2040193953 date "1985-11-01" @default.
- W2040193953 modified "2023-10-02" @default.
- W2040193953 title "Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region" @default.
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- W2040193953 doi "https://doi.org/10.1016/0092-8674(85)90021-2" @default.
- W2040193953 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4075392" @default.
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