FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040201845> ?p ?o ?g. }

• W2040201845 endingPage "685" @default.
• W2040201845 startingPage "679" @default.
• W2040201845 abstract "Measured glomerular filtration rate (mGFR) is the best indicator of renal function in children and adolescents. GFR determination using iohexol clearance has been increasingly accepted and applied in clinical practice because it is accurate, readily available, non-radioactive, safe and is used intravenously even in the presence of renal disease. This study describes the development and evaluation of a semi-automated method for determination of iohexol in human serum using liquid chromatography coupled with electrospray ionization (ESI) tandem mass spectrometry (LC-MS/MS). Iohexol was extracted from serum using a MICROLAB® NIMBUS4 automation robot and supernatant was dried under nitrogen gas and reconstituted in mobile phase. Ioversol was used as the internal standard. Chromatography was performed using a C-8 analytical column (Phenomenex, 3 μm, 50 × 3.0 mm I.D.) at room temperature and a gradient LC method on a Waters 2795 Alliance HT HPLC system. The flow rate was 0.5 mL/min and the retention times were 2.36 min and 2.14 min for iohexol and ioversol, respectively. Detection by MS/MS was achieved using a (Micromass Quattro Micro) tandem mass spectrometer operated in the ESI-positive mode. The multiple-reaction monitoring (MRM) method used ion transitions m/z 821.9 to 803.7 for iohexol and m/z 807.9 to 588.7 for ioversol. Method validation studies were conducted to determine the linearity, accuracy, precision, matrix effects and stability. A method comparison of blinded, residual patient samples was conducted with a well-established method. The method was linear from 7.7 μg/mL to 2000.0 μg/mL. The low limit of quantification and the detection limit were established at 7.7 and 3.0 μg/mL, respectively. Within-run and between-run precisions were found to be < 6% CV and measured values deviated no more than 5% from target concentrations. Carryover and matrix effects were not significant. Comparison to a well-established method showed very good agreement with correlation coefficient of 0.996 for iohexol and 0.993 for GFR/1.73 m2. This method accurately and precisely quantifies iohexol in 50 μL of serum, enabling determination of mGFR by iohexol clearance. The method is highly correlated to a reference method. Use of an automated liquid handler reduces labor-intensive, manual sample preparation steps. The stability of this analyte and the robustness of this assay fit well within our clinical workflow and we have successfully applied this method to determine mGFR in pediatric patients." @default.
• W2040201845 created "2016-06-24" @default.
• W2040201845 creator A5043894861 @default.
• W2040201845 creator A5044187420 @default.
• W2040201845 creator A5051449033 @default.
• W2040201845 creator A5068491758 @default.
• W2040201845 creator A5081899711 @default.
• W2040201845 date "2015-07-01" @default.
• W2040201845 modified "2023-10-02" @default.
• W2040201845 title "Determination of iohexol in human serum by a semi-automated liquid chromatography tandem mass spectrometry method" @default.
• W2040201845 cites W1517420480 @default.
• W2040201845 cites W1992395009 @default.
• W2040201845 cites W2000829819 @default.
• W2040201845 cites W2012083735 @default.
• W2040201845 cites W2015649852 @default.
• W2040201845 cites W2028325321 @default.
• W2040201845 cites W2050226999 @default.
• W2040201845 cites W2072371345 @default.
• W2040201845 cites W2081319665 @default.
• W2040201845 cites W2095140875 @default.
• W2040201845 cites W2132558025 @default.
• W2040201845 cites W2136871445 @default.
• W2040201845 cites W2145748409 @default.
• W2040201845 cites W2152073293 @default.
• W2040201845 cites W2164655181 @default.
• W2040201845 cites W2242104024 @default.
• W2040201845 doi "https://doi.org/10.1016/j.clinbiochem.2015.03.017" @default.
• W2040201845 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25835151" @default.
• W2040201845 hasPublicationYear "2015" @default.
• W2040201845 type Work @default.
• W2040201845 sameAs 2040201845 @default.
• W2040201845 citedByCount "11" @default.
• W2040201845 countsByYear W20402018452016 @default.
• W2040201845 countsByYear W20402018452017 @default.
• W2040201845 countsByYear W20402018452018 @default.
• W2040201845 countsByYear W20402018452020 @default.
• W2040201845 countsByYear W20402018452021 @default.
• W2040201845 countsByYear W20402018452023 @default.
• W2040201845 crossrefType "journal-article" @default.
• W2040201845 hasAuthorship W2040201845A5043894861 @default.
• W2040201845 hasAuthorship W2040201845A5044187420 @default.
• W2040201845 hasAuthorship W2040201845A5051449033 @default.
• W2040201845 hasAuthorship W2040201845A5068491758 @default.
• W2040201845 hasAuthorship W2040201845A5081899711 @default.
• W2040201845 hasConcept C159641895 @default.
• W2040201845 hasConcept C162356407 @default.
• W2040201845 hasConcept C185592680 @default.
• W2040201845 hasConcept C2779093167 @default.
• W2040201845 hasConcept C31827203 @default.
• W2040201845 hasConcept C40684141 @default.
• W2040201845 hasConcept C43617362 @default.
• W2040201845 hasConcept C55493867 @default.
• W2040201845 hasConcept C64489805 @default.
• W2040201845 hasConcept C71043704 @default.
• W2040201845 hasConceptScore W2040201845C159641895 @default.
• W2040201845 hasConceptScore W2040201845C162356407 @default.
• W2040201845 hasConceptScore W2040201845C185592680 @default.
• W2040201845 hasConceptScore W2040201845C2779093167 @default.
• W2040201845 hasConceptScore W2040201845C31827203 @default.
• W2040201845 hasConceptScore W2040201845C40684141 @default.
• W2040201845 hasConceptScore W2040201845C43617362 @default.
• W2040201845 hasConceptScore W2040201845C55493867 @default.
• W2040201845 hasConceptScore W2040201845C64489805 @default.
• W2040201845 hasConceptScore W2040201845C71043704 @default.
• W2040201845 hasIssue "10-11" @default.
• W2040201845 hasLocation W20402018451 @default.
• W2040201845 hasLocation W20402018452 @default.
• W2040201845 hasOpenAccess W2040201845 @default.
• W2040201845 hasPrimaryLocation W20402018451 @default.
• W2040201845 hasRelatedWork W1277187992 @default.
• W2040201845 hasRelatedWork W1863131412 @default.
• W2040201845 hasRelatedWork W1979998703 @default.
• W2040201845 hasRelatedWork W2012075101 @default.
• W2040201845 hasRelatedWork W2021034129 @default.
• W2040201845 hasRelatedWork W2062375825 @default.
• W2040201845 hasRelatedWork W2200897360 @default.
• W2040201845 hasRelatedWork W2385607342 @default.
• W2040201845 hasRelatedWork W2979742567 @default.
• W2040201845 hasRelatedWork W4238213830 @default.
• W2040201845 hasVolume "48" @default.
• W2040201845 isParatext "false" @default.
• W2040201845 isRetracted "false" @default.
• W2040201845 magId "2040201845" @default.
• W2040201845 workType "article" @default.