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- W2040209916 abstract "Methods for knocking out genes and generating transgenic animals are available to study gene functions during embryogenesis. Recently, RNA interference (RNAi), a conserved eukaryotic mechanism in which double-stranded RNA induces sequence-specific degradation of homologous mRNAs, has become a powerful tool for investigating gene function in cells, whole animals, and embryos. Additionally, in vitro mouse limb bud culture is available to study limb development. By microinjection, we established a new method combining the use of RNAi strategies with in vitro mouse limb bud culture to investigate the functions of various genes during embryonic limb development. Using this new method, we found that HspB10 may play a role during skeletal development of limbs. It shows that the combined use of RNAi strategies (via microinjection) with in vitro mouse limb bud culture system may become a technique for investigating gene function in limb development." @default.
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- W2040209916 date "2010-04-19" @default.
- W2040209916 modified "2023-10-10" @default.
- W2040209916 title "Combined use of RNAi strategies with in vitro mouse limb bud culture" @default.
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- W2040209916 doi "https://doi.org/10.1002/jez.b.21345" @default.
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