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• W2040228183 abstract "An improved method is described for the purification of glyoxalase I from rabbit liver. The method involves homogenization, ammonium sulfate precipitation followed by chloroform/ethanol precipitation, affinity chromatography on Blue Dextran-Sepharose 4B and chromatography on Sephadex G-100. The enzyme is specifically eluted from the affinity column by S-hexylglutathione, a competitive inhibitor of the enzyme. This procedure offers a convenient method for obtaining electrophoretically pure glyoxylase I in high yields (60–70%)." @default.
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• W2040228183 date "1981-03-01" @default.
• W2040228183 modified "2023-09-23" @default.
• W2040228183 title "A high-field purification of glyoxalase I from rabbit liver by affinity chromatography on Blue Dextran-Sepharose 4B" @default.
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• W2040228183 doi "https://doi.org/10.1016/0165-022x(81)90061-0" @default.
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