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• W2040244525 abstract "Mycoplasma gallisepticum- and Mycoplasma synoviae-free chickens were infected with 0.2 ml broth culture of M. gallisepticum strain 1226 intra air sac at 3, 14, 18, 28, 42, 49 and 65 days of age. Blood samples were taken 0–5 weeks before infection and 1–6 weeks after infection (depending on age of infection). The antibody response was examined by Western blot. As a control of infection, serum plate agglutination test (SPA), pathological lesions, and presence of Mycoplasma in air sacs were used. Antibodies to p64–67 kDa appeared in all groups of birds on the first week post-infection. Antibodies to p56 were detected from the second week post-challenge if infection was performed at 3 or 14 days of age, while on first week if challenge was done at 18, 28, 42, 49 or 65 days of age. Antibodies to p200, p120, p98, p80, p75, p72, p60, p50, p45, p40, p35, p33, p31, p28, p26, p24 and p22 were also detected. Des poulets indemnes de Mycoplasma gallisepticum et Mycoplasma synoviae ont été infectés avec 0.2 ml d'une culture de M. gallisepticum souche 1226 inoculée dans les sacs aériens a(c) l'âge de 3, 14, 18, 28, 42, 49, et 65 jours. Des échantillons de sang ont été récoltés pendant 5 semaines avant inoculation et de 1 a(c) 6 semaines apre(c)s infection (selon l'âge de l'infection). La réponse en anticorps a été analysée par la technique de Western-blot. Afin de contrôler l'infection, l'agglutination rapide sur lame, la recherche des lésions et la recherche de Mycoplasmes dans les sacs aériens ont été effectués. Des anticorps dirigés vis-a(c)-vis de p64–67 kDa sont apparus dans tous les groupes d' oiseaux de(c)s la premie(c)re semaine apre(c)s infection. Des anticorps spécifiques de p56 sont détectés a(c) partir de la 2e(c)me semaine apre(c)s épreuve si l'infection est réalisée a(c) l' âge de 3 ou 14 jours, ou de(c)s la le(c)re semaine lors de l' infection a(c) 18, 28, 42, 49 ou 65 jours. Des anticorps vis-a(c)-vis de p200, p120, p98, p80, p75, p60, p50, p45, p40, p35, p33, p31, p28, p26, p24, et p22 sont également mis en évidence." @default.
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• W2040244525 date "1997-09-01" @default.
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• W2040244525 title "Immunoblot examination of humoral response of chickens infected with Mycoplasma gallisepticum at various ages" @default.
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• W2040244525 doi "https://doi.org/10.1016/s0147-9571(97)00008-8" @default.
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