Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040247315> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2040247315 endingPage "206" @default.
- W2040247315 startingPage "202" @default.
- W2040247315 abstract "We report the development of a convenient and reliable polymerase chain reaction (PCR)-based microassay for the amplification and detection of specific DNA sequences with potential applications in the diagnostics field. The major features of our system are: (a) the complete system is carried out entirely in the same microtitre well; (b) the PCR is performed in two instead of the traditional three temperatures, thus reducing the time for 35 cycles to under 2 h; (c) the probe is already immobilized onto the solid phase, allowing direct hybridization of the PCR products; (d) one of the two primers is already biotinylated at the 5' end, thus detecting one of the two actual specific products, and (e) the whole process is designed to an enzyme-linked immunosorbent assay (ELISA)-like system for easy use and takes only 3 h, rendering the system particularly suitable for a busy clinical laboratory and automation. The method was successfully applied for the detection of human immunodeficiency virus type 1 (HIV-1) from patient lymphocyte samples." @default.
- W2040247315 created "2016-06-24" @default.
- W2040247315 creator A5038102006 @default.
- W2040247315 creator A5067814157 @default.
- W2040247315 creator A5071420074 @default.
- W2040247315 creator A5077495828 @default.
- W2040247315 date "1995-12-01" @default.
- W2040247315 modified "2023-09-23" @default.
- W2040247315 title "Amplification and non-isotopic detection of specific DNA sequences in a single microtitre well" @default.
- W2040247315 cites W1485928263 @default.
- W2040247315 cites W1517480469 @default.
- W2040247315 cites W1533227151 @default.
- W2040247315 cites W1924412250 @default.
- W2040247315 cites W1946251729 @default.
- W2040247315 cites W1963824741 @default.
- W2040247315 cites W1997206329 @default.
- W2040247315 cites W1999761170 @default.
- W2040247315 cites W2005189491 @default.
- W2040247315 cites W2008606510 @default.
- W2040247315 cites W2032118018 @default.
- W2040247315 cites W2033047970 @default.
- W2040247315 cites W2038075962 @default.
- W2040247315 cites W2047152679 @default.
- W2040247315 cites W2047480444 @default.
- W2040247315 cites W2050717506 @default.
- W2040247315 cites W2055698676 @default.
- W2040247315 cites W2056862882 @default.
- W2040247315 cites W2130600353 @default.
- W2040247315 cites W2148312059 @default.
- W2040247315 cites W2282054059 @default.
- W2040247315 cites W73472175 @default.
- W2040247315 doi "https://doi.org/10.1016/0888-0786(96)87299-4" @default.
- W2040247315 hasPublicationYear "1995" @default.
- W2040247315 type Work @default.
- W2040247315 sameAs 2040247315 @default.
- W2040247315 citedByCount "1" @default.
- W2040247315 crossrefType "journal-article" @default.
- W2040247315 hasAuthorship W2040247315A5038102006 @default.
- W2040247315 hasAuthorship W2040247315A5067814157 @default.
- W2040247315 hasAuthorship W2040247315A5071420074 @default.
- W2040247315 hasAuthorship W2040247315A5077495828 @default.
- W2040247315 hasConcept C104317684 @default.
- W2040247315 hasConcept C153911025 @default.
- W2040247315 hasConcept C159047783 @default.
- W2040247315 hasConcept C185592680 @default.
- W2040247315 hasConcept C204628709 @default.
- W2040247315 hasConcept C3013748606 @default.
- W2040247315 hasConcept C43617362 @default.
- W2040247315 hasConcept C48023723 @default.
- W2040247315 hasConcept C49105822 @default.
- W2040247315 hasConcept C54355233 @default.
- W2040247315 hasConcept C552990157 @default.
- W2040247315 hasConcept C70721500 @default.
- W2040247315 hasConcept C86803240 @default.
- W2040247315 hasConceptScore W2040247315C104317684 @default.
- W2040247315 hasConceptScore W2040247315C153911025 @default.
- W2040247315 hasConceptScore W2040247315C159047783 @default.
- W2040247315 hasConceptScore W2040247315C185592680 @default.
- W2040247315 hasConceptScore W2040247315C204628709 @default.
- W2040247315 hasConceptScore W2040247315C3013748606 @default.
- W2040247315 hasConceptScore W2040247315C43617362 @default.
- W2040247315 hasConceptScore W2040247315C48023723 @default.
- W2040247315 hasConceptScore W2040247315C49105822 @default.
- W2040247315 hasConceptScore W2040247315C54355233 @default.
- W2040247315 hasConceptScore W2040247315C552990157 @default.
- W2040247315 hasConceptScore W2040247315C70721500 @default.
- W2040247315 hasConceptScore W2040247315C86803240 @default.
- W2040247315 hasIssue "4" @default.
- W2040247315 hasLocation W20402473151 @default.
- W2040247315 hasOpenAccess W2040247315 @default.
- W2040247315 hasPrimaryLocation W20402473151 @default.
- W2040247315 hasRelatedWork W1905830411 @default.
- W2040247315 hasRelatedWork W1992766857 @default.
- W2040247315 hasRelatedWork W1994806304 @default.
- W2040247315 hasRelatedWork W2013345504 @default.
- W2040247315 hasRelatedWork W2026999862 @default.
- W2040247315 hasRelatedWork W2036201529 @default.
- W2040247315 hasRelatedWork W2044577327 @default.
- W2040247315 hasRelatedWork W2050169427 @default.
- W2040247315 hasRelatedWork W2053777881 @default.
- W2040247315 hasRelatedWork W2079624236 @default.
- W2040247315 hasRelatedWork W2112542927 @default.
- W2040247315 hasRelatedWork W2180122568 @default.
- W2040247315 hasRelatedWork W2993708391 @default.
- W2040247315 hasRelatedWork W3030069633 @default.
- W2040247315 hasRelatedWork W54738146 @default.
- W2040247315 hasRelatedWork W2087044515 @default.
- W2040247315 hasRelatedWork W2832861468 @default.
- W2040247315 hasRelatedWork W2865076691 @default.
- W2040247315 hasRelatedWork W2878298455 @default.
- W2040247315 hasRelatedWork W3095232238 @default.
- W2040247315 hasVolume "7" @default.
- W2040247315 isParatext "false" @default.
- W2040247315 isRetracted "false" @default.
- W2040247315 magId "2040247315" @default.
- W2040247315 workType "article" @default.