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- W2040252876 abstract "A simple isolation procedure for a photochemically active complex from wildtype cells of the photosynthetic non-sulfur bacterium, Rhodospirillum rubrum, is described. The method involves sucrose density centrifugation of chromatophores equilibrated with a large excess of ascorbate and, subsequently, treated with 1% sodium dodecylsulfate in a concentration of 5.4 g/mmole of bacteriochlorophyll. The resulting brown complex has a mol. wt of about 100 000 and a solubility in aqueous buffer of at least 70 mg/ml. 2.7% of the bacteriochlorophyll of the chromatophores was recovered in this preparation. Stoichiometry appears to hold for P870 to cytochrome c2 (1:1), spirilloxanthin (1:3) and ubiquinone (1:1.7) while cytochrome cc′ was observed in variable amounts. Polyacrylamide gel electrophoresis of this complex using 0.05% sodium dodecylsulfate yielded an even smaller photochemically active fraction (mol. wt approx. 35 000) which contained no cytochrome c2. The amino acid compositions of both fragments are compared." @default.
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- W2040252876 date "1972-06-01" @default.
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- W2040252876 title "Isolation and characteristics of small, soluble photoreactive fragments of Rhodospirillum rubrum" @default.
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- W2040252876 doi "https://doi.org/10.1016/0005-2728(72)90195-8" @default.
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