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- W2040256563 abstract "In order to allow the direct visualization of the cellular trafficking of AAV and thereof derived vectors, we genetically incorporated enhanced green fluorescent protein (GFP) into the AAV capsid by substitution of wild-type VP2 with GFP-VP2 fusion proteins. Our cloning strategy resulted in highly infectious viral preparations minimizing thereby the risk of artefacts. In a first study GFP-VP2-AAV particles were used to investigate nuclear entry. A fast intracellular trafficking was observed and GFP-tagged virions were detected within minutes in the nuclear area of infected cells. In the absence of adenovirus 5 (Ad5) coinfection nuclear translocation was slow and inefficient: no GFP-VP2-AAV particles were detected within the nucleus at 2 hours (h) and 4 h post infection (p.i.). They remained in the perinuclear area and in nuclear membrane invaginations. The colocalization with nuclear invaginations is a phenomenon that was previously observed by Single Virus Tracing measurements (Seisenberger et al. 2001, Science 294. 1929-1932). Nuclear invaginations are long, tubular channels, derived from the nuclear envelope, which extend deeply into the nucleoplasm and are continuous with the cytoplasm. A plausible function of such invaginations is to increase the surface of the nuclear membrane and thereby facilitating the transport through nuclear pores. We are currently investigating the significance of this interesting localization for the AAV infection." @default.
- W2040256563 created "2016-06-24" @default.
- W2040256563 date "2005-05-01" @default.
- W2040256563 modified "2023-09-25" @default.
- W2040256563 title "502. GFP-Tagged Adeno-Associated Viral (AAV) Particles Allow the Study of Cytosolic and Nuclear Trafficking" @default.
- W2040256563 doi "https://doi.org/10.1016/j.ymthe.2005.07.042" @default.
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