Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040258873> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2040258873 endingPage "484" @default.
- W2040258873 startingPage "479" @default.
- W2040258873 abstract "Deoxyribonuclease II has been purified through five fractionation steps from the human lymphoblast cell line K562. Isolation included DEAE-cellulose and heparin-agarose chromatography followed by fractionation on Mono-S, Mono-Q and Superose-12 FPLC columns. In an extension of previous studies, deoxyribonuclease II was found to introduce a much higher proportion of single-strand nicks relative to double-strand breaks into supercoiled DNA than has been reported for linear DNA. Application of DNA sequencing techniques has further revealed a unique resistance of 3' termini to hydrolysis by this enzyme. Deoxyribonuclease II cleaves at every available site along the duplexed portion of a paired oligonucleotide substrate with the exception of the last four nucleotides. Consistent with previous results, this deoxyribonuclease II is active at low pH in the absence of Mg2+ and is not inhibited by EDTA, but complete inhibition is observed with 100 microM Fe3+. Likewise we confirmed the presence of 3'-phosphoryl termini on the DNA cleavage products since they failed to function as primers for DNA synthesis catalyzed by Escherichia coli DNA polymerase I." @default.
- W2040258873 created "2016-06-24" @default.
- W2040258873 creator A5001849897 @default.
- W2040258873 creator A5027447182 @default.
- W2040258873 creator A5065543025 @default.
- W2040258873 creator A5071843900 @default.
- W2040258873 creator A5075717095 @default.
- W2040258873 date "1991-12-01" @default.
- W2040258873 modified "2023-09-26" @default.
- W2040258873 title "Mechanism of action of deoxyribonuclease II from human lymphoblasts" @default.
- W2040258873 cites W1013554086 @default.
- W2040258873 cites W1517004583 @default.
- W2040258873 cites W1534513394 @default.
- W2040258873 cites W1544132533 @default.
- W2040258873 cites W1569970243 @default.
- W2040258873 cites W1592729748 @default.
- W2040258873 cites W1601055202 @default.
- W2040258873 cites W1965268536 @default.
- W2040258873 cites W2038968804 @default.
- W2040258873 cites W2056391548 @default.
- W2040258873 cites W2092260085 @default.
- W2040258873 cites W2095465446 @default.
- W2040258873 cites W2100837269 @default.
- W2040258873 cites W2202410892 @default.
- W2040258873 cites W4323285524 @default.
- W2040258873 doi "https://doi.org/10.1111/j.1432-1033.1991.tb16397.x" @default.
- W2040258873 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1761047" @default.
- W2040258873 hasPublicationYear "1991" @default.
- W2040258873 type Work @default.
- W2040258873 sameAs 2040258873 @default.
- W2040258873 citedByCount "35" @default.
- W2040258873 countsByYear W20402588732012 @default.
- W2040258873 countsByYear W20402588732013 @default.
- W2040258873 countsByYear W20402588732015 @default.
- W2040258873 countsByYear W20402588732016 @default.
- W2040258873 countsByYear W20402588732017 @default.
- W2040258873 countsByYear W20402588732020 @default.
- W2040258873 countsByYear W20402588732023 @default.
- W2040258873 crossrefType "journal-article" @default.
- W2040258873 hasAuthorship W2040258873A5001849897 @default.
- W2040258873 hasAuthorship W2040258873A5027447182 @default.
- W2040258873 hasAuthorship W2040258873A5065543025 @default.
- W2040258873 hasAuthorship W2040258873A5071843900 @default.
- W2040258873 hasAuthorship W2040258873A5075717095 @default.
- W2040258873 hasBestOaLocation W20402588731 @default.
- W2040258873 hasConcept C104317684 @default.
- W2040258873 hasConcept C129312508 @default.
- W2040258873 hasConcept C153911025 @default.
- W2040258873 hasConcept C2756471 @default.
- W2040258873 hasConcept C2777028655 @default.
- W2040258873 hasConcept C2778659741 @default.
- W2040258873 hasConcept C2779377703 @default.
- W2040258873 hasConcept C3017666073 @default.
- W2040258873 hasConcept C512185932 @default.
- W2040258873 hasConcept C552990157 @default.
- W2040258873 hasConcept C55493867 @default.
- W2040258873 hasConcept C86803240 @default.
- W2040258873 hasConceptScore W2040258873C104317684 @default.
- W2040258873 hasConceptScore W2040258873C129312508 @default.
- W2040258873 hasConceptScore W2040258873C153911025 @default.
- W2040258873 hasConceptScore W2040258873C2756471 @default.
- W2040258873 hasConceptScore W2040258873C2777028655 @default.
- W2040258873 hasConceptScore W2040258873C2778659741 @default.
- W2040258873 hasConceptScore W2040258873C2779377703 @default.
- W2040258873 hasConceptScore W2040258873C3017666073 @default.
- W2040258873 hasConceptScore W2040258873C512185932 @default.
- W2040258873 hasConceptScore W2040258873C552990157 @default.
- W2040258873 hasConceptScore W2040258873C55493867 @default.
- W2040258873 hasConceptScore W2040258873C86803240 @default.
- W2040258873 hasIssue "2" @default.
- W2040258873 hasLocation W20402588731 @default.
- W2040258873 hasLocation W20402588732 @default.
- W2040258873 hasOpenAccess W2040258873 @default.
- W2040258873 hasPrimaryLocation W20402588731 @default.
- W2040258873 hasRelatedWork W1557870494 @default.
- W2040258873 hasRelatedWork W1873138889 @default.
- W2040258873 hasRelatedWork W2004554644 @default.
- W2040258873 hasRelatedWork W2017008316 @default.
- W2040258873 hasRelatedWork W2043265428 @default.
- W2040258873 hasRelatedWork W2055839385 @default.
- W2040258873 hasRelatedWork W2070459986 @default.
- W2040258873 hasRelatedWork W2078093047 @default.
- W2040258873 hasRelatedWork W2277612368 @default.
- W2040258873 hasRelatedWork W2416187410 @default.
- W2040258873 hasVolume "202" @default.
- W2040258873 isParatext "false" @default.
- W2040258873 isRetracted "false" @default.
- W2040258873 magId "2040258873" @default.
- W2040258873 workType "article" @default.