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• W2040281650 abstract "High levels of apoptosis and retarded development occur for mouse embryos fertilized in vitro compared to those in vivo. We reported that fertilization environment, in vivo or in vitro, could have influence on the expression of IGF-I or -II mRNA and embryonic apoptosis. These results suggested that higher endogenous growth factor expression should be beneficial for apoptosis suppression by autocrine pathway. Therefore, the objectives of the present study were 1) to investigate the apoptotic rates for embryos fertilized in vivo and in vitro and 2) to examine whether exogenous IGF-I and -II in the in vitro culture medium overcome apoptosis occurrence. The effects of exogenous IGF-I or/and -II in IVF and culture media were examined on embryo development and apoptosis expression. BD F1 mice, 5-6 weeks old, were superovulated by 5 IU PMSG followed 48 h later by 5 IU HCG injection. Some of them were paired overnight with males and others were not paired to perform IVF. The presence of a copulation plug was regarded as day 0 of pregnancy. Oocyte-cumulus complexes for IVF were collected from the oviduct 14-16 h after HCG injection. IVF was performed using sperm collected from 10-weeks aged males in a 20 ul of mMTF containing BSA (16 mg/ml) in the presence or absence of IGF-I and -II (10 ng/ml). Zygotes derived from IVF were cultured in a 20 ul of mMTF containing BSA (4 mg/ml) in which IGFs were supplemented at the same concentration(s) as IVF medium by day 5. Two-cell embryos fertilized in vivo were collected by flushing oviducts on day 1, and then cultured by day 5. Apoptotic cells of day 5 blastocysts were identified using TUNEL labeling and Hoechst counterstain to evaluate DNA fragmentation, total cell, and nuclear morphology, respectively. As shown in table, the fertilization rates were significantly higher in IGFs-treated groups than an untreated group (P < 0.05). Although the developments to the blastocyst stage in in vitro groups were lower than in vivo group, IGFs-treated groups were higher than an untreated group. However, no effect was observed between individual and combination of IGF-I and -II. Specially, total cell numbers and apoptosis occurrences obtained from IGFs-treated in vitro groups tended to be similar to those in in vivo group. Tabled 1 The embryos treated with IGFs showed higher fertilization rate and total cell number as well as lower apoptotic index than those not treated group. Although embryo development was low, the embryos in IGFs-treated groups showed similar total cell number and apoptotic index to those in in vivo group. This study suggests that IGFs should have influence on not only embryo development but also embryonic apoptosis. In conclusion, the addition of IGFs was hence overcome an apoptosis in embryos fertilized in vitro as those in vivo." @default.
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• W2040281650 date "2005-09-01" @default.
• W2040281650 modified "2023-10-14" @default.
• W2040281650 title "Effects of IGF-I and -II to Apoptosis Expression in Pre-Implantation Mouse Embryos" @default.
• W2040281650 doi "https://doi.org/10.1016/j.fertnstert.2005.07.1155" @default.
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