FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040282068> ?p ?o ?g. }

• W2040282068 endingPage "507" @default.
• W2040282068 startingPage "502" @default.
• W2040282068 abstract "Boar sperm TyrP32 is a 32-kDa tyrosine-phosphorylated protein that increases during the capacitation and acrosome reaction and during cryocapacitation. However, it is still unclear whether the increase in TyrP32 is an event that is limited to the process of sperm fertilization, including cryocapacitation. The aims of the present study were to demonstrate that TyrP32 is increased in dead spermatozoa after freeze-thawing without a cryoprotectant and to find the causal factors for this increase. Washed spermatozoa were resuspended in a salt solution and then frozen. The frozen samples were rapidly thawed in a warm water bath and then used for sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE)/Western blotting to detect TyrP32, SDS-PAGE/silver staining of sperm proteins and staining of acrosomal contents with fluorescein isothiocyanate (FITC)-conjugated peanut agglutinin (PNA). In the samples before freezing, TyrP32 was barely detectable, and the distribution of the acrosomal contents was normal in most spermatozoa. One cycle of freeze-thawing induced an increase in TyrP32, a decrease in major sperm proteins and disorder in the acrosomal contents. However, the addition of a protease inhibitor (APMSF, 1 mM) suppressed the increase in TyrP32 and the decrease in the major sperm proteins, although it did not have any influence on the disorder in the acrosomal contents. Additionally, the spermatozoa did not exhibit any flagellar movement after freeze-thawing, which showed that almost all of them were dead. These results indicate that TyrP32 can show a protease-dependent increase in dead spermatozoa after freeze-thawing without a cryoprotectant even though the dead spermatozoa do not undergo cryocapacitation." @default.
• W2040282068 created "2016-06-24" @default.
• W2040282068 creator A5015778891 @default.
• W2040282068 creator A5021514657 @default.
• W2040282068 creator A5049554541 @default.
• W2040282068 date "2008-01-01" @default.
• W2040282068 modified "2023-10-16" @default.
• W2040282068 title "A 32-kDa Tyrosine-phosphorylated Protein Shows a Protease-dependent Increase in Dead Boar Spermatozoa" @default.
• W2040282068 cites W1493322593 @default.
• W2040282068 cites W1705202326 @default.
• W2040282068 cites W1965974282 @default.
• W2040282068 cites W1978041128 @default.
• W2040282068 cites W1987900787 @default.
• W2040282068 cites W1995362740 @default.
• W2040282068 cites W2015373368 @default.
• W2040282068 cites W2019031546 @default.
• W2040282068 cites W2062437600 @default.
• W2040282068 cites W2064503589 @default.
• W2040282068 cites W2066417517 @default.
• W2040282068 cites W2073225515 @default.
• W2040282068 cites W2081990324 @default.
• W2040282068 cites W2094948816 @default.
• W2040282068 cites W2099158671 @default.
• W2040282068 cites W2123407654 @default.
• W2040282068 cites W2138840469 @default.
• W2040282068 cites W2140439130 @default.
• W2040282068 cites W2145559156 @default.
• W2040282068 cites W2157486476 @default.
• W2040282068 cites W2165742171 @default.
• W2040282068 doi "https://doi.org/10.1262/jrd.20021" @default.
• W2040282068 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18787309" @default.
• W2040282068 hasPublicationYear "2008" @default.
• W2040282068 type Work @default.
• W2040282068 sameAs 2040282068 @default.
• W2040282068 citedByCount "17" @default.
• W2040282068 countsByYear W20402820682012 @default.
• W2040282068 countsByYear W20402820682013 @default.
• W2040282068 countsByYear W20402820682014 @default.
• W2040282068 countsByYear W20402820682018 @default.
• W2040282068 countsByYear W20402820682019 @default.
• W2040282068 countsByYear W20402820682020 @default.
• W2040282068 countsByYear W20402820682021 @default.
• W2040282068 countsByYear W20402820682022 @default.
• W2040282068 countsByYear W20402820682023 @default.
• W2040282068 crossrefType "journal-article" @default.
• W2040282068 hasAuthorship W2040282068A5015778891 @default.
• W2040282068 hasAuthorship W2040282068A5021514657 @default.
• W2040282068 hasAuthorship W2040282068A5049554541 @default.
• W2040282068 hasBestOaLocation W20402820681 @default.
• W2040282068 hasConcept C105702510 @default.
• W2040282068 hasConcept C116084860 @default.
• W2040282068 hasConcept C11960822 @default.
• W2040282068 hasConcept C138885662 @default.
• W2040282068 hasConcept C16685009 @default.
• W2040282068 hasConcept C179933525 @default.
• W2040282068 hasConcept C181199279 @default.
• W2040282068 hasConcept C185592680 @default.
• W2040282068 hasConcept C196843134 @default.
• W2040282068 hasConcept C27206212 @default.
• W2040282068 hasConcept C2776714187 @default.
• W2040282068 hasConcept C2777447553 @default.
• W2040282068 hasConcept C2777553839 @default.
• W2040282068 hasConcept C2778252442 @default.
• W2040282068 hasConcept C2779995066 @default.
• W2040282068 hasConcept C2780301668 @default.
• W2040282068 hasConcept C2780761556 @default.
• W2040282068 hasConcept C2781087480 @default.
• W2040282068 hasConcept C2781292093 @default.
• W2040282068 hasConcept C54355233 @default.
• W2040282068 hasConcept C55493867 @default.
• W2040282068 hasConcept C59822182 @default.
• W2040282068 hasConcept C68528507 @default.
• W2040282068 hasConcept C71924100 @default.
• W2040282068 hasConcept C74864618 @default.
• W2040282068 hasConcept C86803240 @default.
• W2040282068 hasConcept C88972607 @default.
• W2040282068 hasConcept C95444343 @default.
• W2040282068 hasConceptScore W2040282068C105702510 @default.
• W2040282068 hasConceptScore W2040282068C116084860 @default.
• W2040282068 hasConceptScore W2040282068C11960822 @default.
• W2040282068 hasConceptScore W2040282068C138885662 @default.
• W2040282068 hasConceptScore W2040282068C16685009 @default.
• W2040282068 hasConceptScore W2040282068C179933525 @default.
• W2040282068 hasConceptScore W2040282068C181199279 @default.
• W2040282068 hasConceptScore W2040282068C185592680 @default.
• W2040282068 hasConceptScore W2040282068C196843134 @default.
• W2040282068 hasConceptScore W2040282068C27206212 @default.
• W2040282068 hasConceptScore W2040282068C2776714187 @default.
• W2040282068 hasConceptScore W2040282068C2777447553 @default.
• W2040282068 hasConceptScore W2040282068C2777553839 @default.
• W2040282068 hasConceptScore W2040282068C2778252442 @default.
• W2040282068 hasConceptScore W2040282068C2779995066 @default.
• W2040282068 hasConceptScore W2040282068C2780301668 @default.
• W2040282068 hasConceptScore W2040282068C2780761556 @default.
• W2040282068 hasConceptScore W2040282068C2781087480 @default.
• W2040282068 hasConceptScore W2040282068C2781292093 @default.
• W2040282068 hasConceptScore W2040282068C54355233 @default.
• W2040282068 hasConceptScore W2040282068C55493867 @default.

• next