Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040291293> ?p ?o ?g. }
- W2040291293 endingPage "78" @default.
- W2040291293 startingPage "72" @default.
- W2040291293 abstract "Natural killer (NK) cells produce multiple cytokines with potential immune regulatory roles. We standardised a whole-blood flow cytometry method to visualise intracellular cytokine production by NK cells for the study of NK cell biology and for clinical monitoring.With a three-colour fluorescent labelling technique, specific cytokine production by NK or T cells was visualised directly in whole blood in the same sample after stimulation by phorbol 12-myristate 13-acetate (PMA) and ionomycin and by electronically gating on the CD3-ve/CD56+ve NK population or on the CD3+/CD56+ NK-T-cell population.Detectable levels of tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) but not of interleukin-2 (IL-2) or IL-4 were easily observed in NK cells. The visualisation of the cytokine production by NK cells was dependent on the addition of a Golgi transport inhibitor, Brefeldin A. Other known stimuli for NK cells (IL-2 and CD16 monoclonal antibody and incubation with K562, the NK-sensitive cell line) promoted IFN-gamma and TNF-alpha production in NK cells to a lesser extent than did PMA and ionomycin stimulation.This whole-blood flow cytometric assay appears to be an useful and easy method to examine cytokine production by NK cells and/or by CD3+CD56+ NK-T lymphocytes in patients with relevant diseases." @default.
- W2040291293 created "2016-06-24" @default.
- W2040291293 creator A5001503641 @default.
- W2040291293 creator A5018293998 @default.
- W2040291293 creator A5035383163 @default.
- W2040291293 creator A5043049216 @default.
- W2040291293 creator A5047574164 @default.
- W2040291293 creator A5050696925 @default.
- W2040291293 creator A5064706934 @default.
- W2040291293 date "2000-01-01" @default.
- W2040291293 modified "2023-10-16" @default.
- W2040291293 title "Flow cytometric visualisation of cytokine production by CD3-CD56+ NK cells and CD3+CD56+ NK-T cells in whole blood" @default.
- W2040291293 cites W1504887220 @default.
- W2040291293 cites W1564959198 @default.
- W2040291293 cites W1580335186 @default.
- W2040291293 cites W1593813009 @default.
- W2040291293 cites W1645991544 @default.
- W2040291293 cites W1674854702 @default.
- W2040291293 cites W1976535486 @default.
- W2040291293 cites W1977608747 @default.
- W2040291293 cites W1979343444 @default.
- W2040291293 cites W1999002546 @default.
- W2040291293 cites W2025005913 @default.
- W2040291293 cites W2028240889 @default.
- W2040291293 cites W2030226186 @default.
- W2040291293 cites W2037723969 @default.
- W2040291293 cites W2052054743 @default.
- W2040291293 cites W2053929975 @default.
- W2040291293 cites W2055669282 @default.
- W2040291293 cites W2057567758 @default.
- W2040291293 cites W2059428912 @default.
- W2040291293 cites W2059490409 @default.
- W2040291293 cites W2064558165 @default.
- W2040291293 cites W2069340283 @default.
- W2040291293 cites W2074221799 @default.
- W2040291293 cites W2074846327 @default.
- W2040291293 cites W2085527849 @default.
- W2040291293 cites W2087296395 @default.
- W2040291293 cites W2088617902 @default.
- W2040291293 cites W2118205899 @default.
- W2040291293 cites W2118376550 @default.
- W2040291293 cites W2127022844 @default.
- W2040291293 cites W2133114658 @default.
- W2040291293 cites W2140288892 @default.
- W2040291293 cites W2141672711 @default.
- W2040291293 cites W2155782151 @default.
- W2040291293 cites W2160520392 @default.
- W2040291293 cites W2268886790 @default.
- W2040291293 cites W2290893624 @default.
- W2040291293 cites W4313343422 @default.
- W2040291293 doi "https://doi.org/10.1002/(sici)1097-0320(20000101)39:1<72::aid-cyto10>3.0.co;2-r" @default.
- W2040291293 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10655565" @default.
- W2040291293 hasPublicationYear "2000" @default.
- W2040291293 type Work @default.
- W2040291293 sameAs 2040291293 @default.
- W2040291293 citedByCount "54" @default.
- W2040291293 countsByYear W20402912932012 @default.
- W2040291293 countsByYear W20402912932013 @default.
- W2040291293 countsByYear W20402912932014 @default.
- W2040291293 countsByYear W20402912932015 @default.
- W2040291293 countsByYear W20402912932018 @default.
- W2040291293 countsByYear W20402912932019 @default.
- W2040291293 countsByYear W20402912932020 @default.
- W2040291293 countsByYear W20402912932022 @default.
- W2040291293 crossrefType "journal-article" @default.
- W2040291293 hasAuthorship W2040291293A5001503641 @default.
- W2040291293 hasAuthorship W2040291293A5018293998 @default.
- W2040291293 hasAuthorship W2040291293A5035383163 @default.
- W2040291293 hasAuthorship W2040291293A5043049216 @default.
- W2040291293 hasAuthorship W2040291293A5047574164 @default.
- W2040291293 hasAuthorship W2040291293A5050696925 @default.
- W2040291293 hasAuthorship W2040291293A5064706934 @default.
- W2040291293 hasConcept C114684123 @default.
- W2040291293 hasConcept C129374314 @default.
- W2040291293 hasConcept C149532602 @default.
- W2040291293 hasConcept C153911025 @default.
- W2040291293 hasConcept C154317977 @default.
- W2040291293 hasConcept C167672396 @default.
- W2040291293 hasConcept C183315844 @default.
- W2040291293 hasConcept C202751555 @default.
- W2040291293 hasConcept C203014093 @default.
- W2040291293 hasConcept C2776090121 @default.
- W2040291293 hasConcept C2776989580 @default.
- W2040291293 hasConcept C2778690821 @default.
- W2040291293 hasConcept C2781462264 @default.
- W2040291293 hasConcept C28651165 @default.
- W2040291293 hasConcept C2908647359 @default.
- W2040291293 hasConcept C553184892 @default.
- W2040291293 hasConcept C55493867 @default.
- W2040291293 hasConcept C71924100 @default.
- W2040291293 hasConcept C79879829 @default.
- W2040291293 hasConcept C86803240 @default.
- W2040291293 hasConcept C8891405 @default.
- W2040291293 hasConcept C95444343 @default.
- W2040291293 hasConcept C99454951 @default.
- W2040291293 hasConceptScore W2040291293C114684123 @default.
- W2040291293 hasConceptScore W2040291293C129374314 @default.
- W2040291293 hasConceptScore W2040291293C149532602 @default.