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- W2040321314 abstract "USERIA is a modification of radioimmunoassay and enzyme-liked immunosorbent assay. When compared to these assays, USERIA is 60- and 10-fold, respectively, more sensitive for the detection of adducts in DNA modified by the chemical carcinogen, 2-acetylaminofluorene (AAF). The specific antigen-antibody reaction is enzymatically amplified by an anti-IgG alkaline phosphatase conjugate which converts the substrate, [3H]adenosine-5'-monophosphate, to [3H]adenosine. As few as 2 fmol of AAF-DNA adducts can be detected by a competitive USERIA assay and less than 3 fmol of the specific adduct, N-(deoxyguanosine-8-yl)-AAF, can be detected using a non-competitive USERIA approach. The sensitivity of USERIA should aid efforts to measure carcinogen-DNA adducts in biological specimen samples from humans and experimental animals." @default.
- W2040321314 created "2016-06-24" @default.
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- W2040321314 date "1980-01-01" @default.
- W2040321314 modified "2023-09-23" @default.
- W2040321314 title "Ultrasensitive enzymatic radioimmunoassay (USERIA) detects femtomoles of acetylaminofluorene-DNA adducts" @default.
- W2040321314 doi "https://doi.org/10.1093/carcin/1.5.455" @default.
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