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• W2040322479 abstract "The isolation and characterization of a myogenic cell line from C57BL/6J/dydy mice is described. This line (DyA4) maintains the morphological, biochemical and elec-trophysiological characteristics of the primary cultured cells, at least for 20 passages. The cells actively divide as long as they are subcultured in media supplemented with horse serum and embryo extract. If the cells are not subcultured for a few days, they fuse into multinucleated contracting myotubes, which readily synthesize specific muscle products such as acetylcholinesterase and acetylcholine receptor. This dystrophic cell line expresses in vitro the same altered phenotype that is characteristic of dystrophic muscle cells in primary cultures, namely reduced acetylcholine sensitivity and reduced acetylcholine receptor expression. Because they can be grown in large amounts, and represent a pure muscle cell population which express an altered phenotype in an in vitro aneural avascular environment, DyA4 cells provide a very useful model system for investigating the pathogenesis of murinc muscular dystrophy." @default.
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• W2040322479 date "1986-10-01" @default.
• W2040322479 modified "2023-09-24" @default.
• W2040322479 title "A muscle cell line from dystrophic mice expressing an altered phenotype in vitro" @default.
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• W2040322479 doi "https://doi.org/10.1111/j.1432-0436.1986.tb00571.x" @default.
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