Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040335792> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2040335792 endingPage "302" @default.
- W2040335792 startingPage "293" @default.
- W2040335792 abstract "Fumarase (fumarate hydratase EC 4.2.1.2.) from Pseudomonas putida was purified 790 times the specific activity of the crude extract. A molecular weight of 166,000 was obtained by sucrose density gradient centrifugation. Low concentrations of thiol inhibitors (10−4m) did not inhibit the enzyme, but preincubation of the enzyme with high concentrations (0.05 m) of the inhibitors markedly decreased enzyme activity. The catalysis of the hydration of fumarate and dehydration of l-malate by fumarase was measured spectrophotometrically over a range of substrate concentrations of 2.5 × 10−4 −5 × 10−3m for l-malate and 10−4 −10−3m for fumarate. The studies were conducted over a pH range of 5.5–9.5 in 0.05 m sodium phosphate buffer at 30 °. The pH dependence of maximum initial velocity exhibited maxima at 6.9 with fumarate, and 8.1 with malate, as substrates. The same ionization constant values were demonstrated for the two essential groups in the free enzyme for either substrate which indicated that the same enzymatic site converts l-malate to fumarate and fumarate to l-malate. The ionization constants for the enzyme-substrate complex agree closely with the ones obtained for the porcine enzyme and indicate the mechanism of action of bacterial enzyme to be identical to the porcine enzyme. Michaelis constants and maximum initial velocity constants were decreased by increasing concentrations of phosphate buffer. It is thus possible that phosphate influences the ionization of groups in the active site of the enzyme or slightly changes the configuration of the site." @default.
- W2040335792 created "2016-06-24" @default.
- W2040335792 creator A5024124503 @default.
- W2040335792 creator A5034589435 @default.
- W2040335792 creator A5063398027 @default.
- W2040335792 date "1970-11-01" @default.
- W2040335792 modified "2023-10-13" @default.
- W2040335792 title "Purification and characterization of fumarase from Pseudomonas putida" @default.
- W2040335792 cites W10451710 @default.
- W2040335792 cites W1495273590 @default.
- W2040335792 cites W1611482599 @default.
- W2040335792 cites W1914630957 @default.
- W2040335792 cites W196748724 @default.
- W2040335792 cites W1982503123 @default.
- W2040335792 cites W2016266841 @default.
- W2040335792 cites W2017481350 @default.
- W2040335792 cites W2018649933 @default.
- W2040335792 cites W2021754494 @default.
- W2040335792 cites W2022808890 @default.
- W2040335792 cites W2026810604 @default.
- W2040335792 cites W2044809585 @default.
- W2040335792 cites W2063638273 @default.
- W2040335792 cites W2111301002 @default.
- W2040335792 cites W2324871183 @default.
- W2040335792 cites W2326167715 @default.
- W2040335792 cites W2327474210 @default.
- W2040335792 doi "https://doi.org/10.1016/0003-9861(70)90135-9" @default.
- W2040335792 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5529637" @default.
- W2040335792 hasPublicationYear "1970" @default.
- W2040335792 type Work @default.
- W2040335792 sameAs 2040335792 @default.
- W2040335792 citedByCount "8" @default.
- W2040335792 countsByYear W20403357922012 @default.
- W2040335792 countsByYear W20403357922015 @default.
- W2040335792 crossrefType "journal-article" @default.
- W2040335792 hasAuthorship W2040335792A5024124503 @default.
- W2040335792 hasAuthorship W2040335792A5034589435 @default.
- W2040335792 hasAuthorship W2040335792A5063398027 @default.
- W2040335792 hasConcept C106302636 @default.
- W2040335792 hasConcept C132901563 @default.
- W2040335792 hasConcept C13965031 @default.
- W2040335792 hasConcept C181199279 @default.
- W2040335792 hasConcept C185592680 @default.
- W2040335792 hasConcept C18903297 @default.
- W2040335792 hasConcept C2777289219 @default.
- W2040335792 hasConcept C2780543182 @default.
- W2040335792 hasConcept C28212737 @default.
- W2040335792 hasConcept C43617362 @default.
- W2040335792 hasConcept C55493867 @default.
- W2040335792 hasConcept C64915919 @default.
- W2040335792 hasConcept C86803240 @default.
- W2040335792 hasConceptScore W2040335792C106302636 @default.
- W2040335792 hasConceptScore W2040335792C132901563 @default.
- W2040335792 hasConceptScore W2040335792C13965031 @default.
- W2040335792 hasConceptScore W2040335792C181199279 @default.
- W2040335792 hasConceptScore W2040335792C185592680 @default.
- W2040335792 hasConceptScore W2040335792C18903297 @default.
- W2040335792 hasConceptScore W2040335792C2777289219 @default.
- W2040335792 hasConceptScore W2040335792C2780543182 @default.
- W2040335792 hasConceptScore W2040335792C28212737 @default.
- W2040335792 hasConceptScore W2040335792C43617362 @default.
- W2040335792 hasConceptScore W2040335792C55493867 @default.
- W2040335792 hasConceptScore W2040335792C64915919 @default.
- W2040335792 hasConceptScore W2040335792C86803240 @default.
- W2040335792 hasIssue "1" @default.
- W2040335792 hasLocation W20403357921 @default.
- W2040335792 hasLocation W20403357922 @default.
- W2040335792 hasOpenAccess W2040335792 @default.
- W2040335792 hasPrimaryLocation W20403357921 @default.
- W2040335792 hasRelatedWork W148367721 @default.
- W2040335792 hasRelatedWork W1486688369 @default.
- W2040335792 hasRelatedWork W163307210 @default.
- W2040335792 hasRelatedWork W1993483068 @default.
- W2040335792 hasRelatedWork W2051397159 @default.
- W2040335792 hasRelatedWork W2078732564 @default.
- W2040335792 hasRelatedWork W2168420088 @default.
- W2040335792 hasRelatedWork W2435237618 @default.
- W2040335792 hasRelatedWork W341996559 @default.
- W2040335792 hasRelatedWork W4206867804 @default.
- W2040335792 hasVolume "141" @default.
- W2040335792 isParatext "false" @default.
- W2040335792 isRetracted "false" @default.
- W2040335792 magId "2040335792" @default.
- W2040335792 workType "article" @default.