Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040339956> ?p ?o ?g. }
- W2040339956 endingPage "208" @default.
- W2040339956 startingPage "201" @default.
- W2040339956 abstract "Catalytic and inhibitor binding properties of bovine alpha-chymotrypsin, in which the Met-192 residue has been converted by treatment with chloramine T to the sulfoxide derivative (Met(O)192 alpha-chymotrypsin), have been examined relative to the native enzyme (alpha-chymotrypsin), between pH 4.5 and 8.0 (mu = 0.1), and/or 5.0 degrees C and 40.0 degrees C. Values of kcat, k+2 and/or k+3 for the hydrolysis of all the substrates examined (i.e., tMetAcONp, ZAlaONp, ZLeuONp, ZLysONp and ZTyrONp) catalyzed by native and Met(O)192 alpha-chymotrypsin are similar, as well as values of Km for the hydrolysis of ZLeuONp, ZLysONp and ZTyrONp. On the other hand, Ks and Km values for the hydrolysis of ZAlaONp and tMetAcONp are decreased by about 5-fold. Met-192 oxidation does not affect the kinetic and thermodynamic parameters for the (de)stabilization of the complex formed between the proteinase and the bovine basic pancreatic trypsin inhibitor. On the other hand, the recognition process between between alpha-chymotrypsin and the recombinant proteinase inhibitor eglin c from the leech Hirudo medicinalis is influenced by the oxidation event. Considering known molecular models, the observed catalytic and inhibitor binding properties of native and Met(O)192 alpha-chymotrypsin were related to the inferred stereochemistry of the proteinase-substrate and proteinase-inhibitor contact region(s)." @default.
- W2040339956 created "2016-06-24" @default.
- W2040339956 creator A5001056492 @default.
- W2040339956 creator A5002157417 @default.
- W2040339956 creator A5007314231 @default.
- W2040339956 creator A5011714668 @default.
- W2040339956 creator A5012594629 @default.
- W2040339956 creator A5025923040 @default.
- W2040339956 creator A5038438275 @default.
- W2040339956 creator A5049565862 @default.
- W2040339956 creator A5060258601 @default.
- W2040339956 date "1993-02-01" @default.
- W2040339956 modified "2023-10-14" @default.
- W2040339956 title "Selective oxidation of Met-192 in bovine α-chymotrypsin. Effect on catalytic and inhibitor binding properties" @default.
- W2040339956 cites W1572262714 @default.
- W2040339956 cites W1582753604 @default.
- W2040339956 cites W1632690303 @default.
- W2040339956 cites W1754330606 @default.
- W2040339956 cites W1838343609 @default.
- W2040339956 cites W1884187048 @default.
- W2040339956 cites W1946659263 @default.
- W2040339956 cites W1966041739 @default.
- W2040339956 cites W1969222787 @default.
- W2040339956 cites W1975962599 @default.
- W2040339956 cites W1980337146 @default.
- W2040339956 cites W1986741205 @default.
- W2040339956 cites W1993853000 @default.
- W2040339956 cites W2000888973 @default.
- W2040339956 cites W2022416858 @default.
- W2040339956 cites W2034312629 @default.
- W2040339956 cites W2037773161 @default.
- W2040339956 cites W2042324397 @default.
- W2040339956 cites W2046452071 @default.
- W2040339956 cites W2046893750 @default.
- W2040339956 cites W2049838860 @default.
- W2040339956 cites W2051839959 @default.
- W2040339956 cites W2054123026 @default.
- W2040339956 cites W2068582249 @default.
- W2040339956 cites W2081058810 @default.
- W2040339956 cites W2081519659 @default.
- W2040339956 cites W2084301904 @default.
- W2040339956 cites W2091043009 @default.
- W2040339956 cites W2092331199 @default.
- W2040339956 cites W2100837269 @default.
- W2040339956 cites W2105987887 @default.
- W2040339956 cites W2157427549 @default.
- W2040339956 cites W2177731497 @default.
- W2040339956 cites W2209250776 @default.
- W2040339956 cites W2333435509 @default.
- W2040339956 cites W2462388258 @default.
- W2040339956 cites W86486060 @default.
- W2040339956 doi "https://doi.org/10.1016/0167-4838(93)90214-c" @default.
- W2040339956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8431470" @default.
- W2040339956 hasPublicationYear "1993" @default.
- W2040339956 type Work @default.
- W2040339956 sameAs 2040339956 @default.
- W2040339956 citedByCount "5" @default.
- W2040339956 crossrefType "journal-article" @default.
- W2040339956 hasAuthorship W2040339956A5001056492 @default.
- W2040339956 hasAuthorship W2040339956A5002157417 @default.
- W2040339956 hasAuthorship W2040339956A5007314231 @default.
- W2040339956 hasAuthorship W2040339956A5011714668 @default.
- W2040339956 hasAuthorship W2040339956A5012594629 @default.
- W2040339956 hasAuthorship W2040339956A5025923040 @default.
- W2040339956 hasAuthorship W2040339956A5038438275 @default.
- W2040339956 hasAuthorship W2040339956A5049565862 @default.
- W2040339956 hasAuthorship W2040339956A5060258601 @default.
- W2040339956 hasConcept C181199279 @default.
- W2040339956 hasConcept C185592680 @default.
- W2040339956 hasConcept C2778401398 @default.
- W2040339956 hasConcept C2780340462 @default.
- W2040339956 hasConcept C41183919 @default.
- W2040339956 hasConcept C55493867 @default.
- W2040339956 hasConcept C56856141 @default.
- W2040339956 hasConcept C71240020 @default.
- W2040339956 hasConcept C94412978 @default.
- W2040339956 hasConceptScore W2040339956C181199279 @default.
- W2040339956 hasConceptScore W2040339956C185592680 @default.
- W2040339956 hasConceptScore W2040339956C2778401398 @default.
- W2040339956 hasConceptScore W2040339956C2780340462 @default.
- W2040339956 hasConceptScore W2040339956C41183919 @default.
- W2040339956 hasConceptScore W2040339956C55493867 @default.
- W2040339956 hasConceptScore W2040339956C56856141 @default.
- W2040339956 hasConceptScore W2040339956C71240020 @default.
- W2040339956 hasConceptScore W2040339956C94412978 @default.
- W2040339956 hasIssue "2-3" @default.
- W2040339956 hasLocation W20403399561 @default.
- W2040339956 hasLocation W20403399562 @default.
- W2040339956 hasOpenAccess W2040339956 @default.
- W2040339956 hasPrimaryLocation W20403399561 @default.
- W2040339956 hasRelatedWork W2028304230 @default.
- W2040339956 hasRelatedWork W2051109782 @default.
- W2040339956 hasRelatedWork W2097392642 @default.
- W2040339956 hasRelatedWork W2130694116 @default.
- W2040339956 hasRelatedWork W2362799233 @default.
- W2040339956 hasRelatedWork W2393808451 @default.
- W2040339956 hasRelatedWork W403672253 @default.
- W2040339956 hasRelatedWork W987493211 @default.