FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040343062> ?p ?o ?g. }

• W2040343062 endingPage "e80776" @default.
• W2040343062 startingPage "e80776" @default.
• W2040343062 abstract "Embryonic stem cells (ESCs) are characterized by two remarkable peculiarities: the capacity to propagate as undifferentiated cells (self-renewal) and the ability to differentiate in ectoderm, endoderm, and mesoderm derivatives (pluripotency). Although the majority of ESCs divide without losing the pluripotency, it has become evident that ESC cultures consists of multiple cell populations highlighted by the expression of early germ lineage markers during spontaneous differentiation. Hence, the identification and characterization of ESCs subpopulations represents an efficient approach to improve the comprehension of correlation between gene expression and cell specification status. To study markers of ESCs heterogeneity, we developed an analysis pipeline which can automatically process images of stem cell colonies in optical microscopy. The question we try to address is to find out the statistically significant preferred locations of the marked cells. We tested our algorithm on a set of images of stem cell colonies to analyze the expression pattern of the Zscan4 gene, which was an elite candidate gene to be studied because it is specifically expressed in subpopulation of ESCs. To validate the proposed method we analyzed the behavior of control genes whose pattern had been associated to biological status such as differentiation (EndoA), pluripotency (Pou5f1), and pluripotency fluctuation (Nanog). We found that Zscan4 is not uniformly expressed inside a stem cell colony, and that it tends to be expressed towards the center of the colony, moreover cells expressing Zscan4 cluster each other. This is of significant importance because it allows us to hypothesize a biological status where the cells expressing Zscan4 are preferably associated to the inner of colonies suggesting pluripotent cell status features, and the clustering between themselves suggests either a colony paracrine effect or an early phase of cell specification through proliferation. Also, the analysis on the control genes showed that they behave as expected." @default.
• W2040343062 created "2016-06-24" @default.
• W2040343062 creator A5014115464 @default.
• W2040343062 creator A5068855074 @default.
• W2040343062 creator A5075960896 @default.
• W2040343062 creator A5085527072 @default.
• W2040343062 date "2013-12-09" @default.
• W2040343062 modified "2023-09-25" @default.
• W2040343062 title "Automated Identification and Location Analysis of Marked Stem Cells Colonies in Optical Microscopy Images" @default.
• W2040343062 cites W1608762409 @default.
• W2040343062 cites W1809476640 @default.
• W2040343062 cites W1964076580 @default.
• W2040343062 cites W1977757945 @default.
• W2040343062 cites W1981398520 @default.
• W2040343062 cites W2001002651 @default.
• W2040343062 cites W2001412060 @default.
• W2040343062 cites W2001432314 @default.
• W2040343062 cites W2005808461 @default.
• W2040343062 cites W2015357416 @default.
• W2040343062 cites W2025818287 @default.
• W2040343062 cites W2034923684 @default.
• W2040343062 cites W2036326229 @default.
• W2040343062 cites W2041098333 @default.
• W2040343062 cites W2069236071 @default.
• W2040343062 cites W2093834886 @default.
• W2040343062 cites W2100383758 @default.
• W2040343062 cites W2105830710 @default.
• W2040343062 cites W2123114670 @default.
• W2040343062 cites W2126851387 @default.
• W2040343062 cites W2137069423 @default.
• W2040343062 cites W2139046195 @default.
• W2040343062 cites W2140099710 @default.
• W2040343062 cites W2159386603 @default.
• W2040343062 cites W2164822588 @default.
• W2040343062 cites W2505399031 @default.
• W2040343062 cites W4239726656 @default.
• W2040343062 doi "https://doi.org/10.1371/journal.pone.0080776" @default.
• W2040343062 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3857180" @default.
• W2040343062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24349016" @default.
• W2040343062 hasPublicationYear "2013" @default.
• W2040343062 type Work @default.
• W2040343062 sameAs 2040343062 @default.
• W2040343062 citedByCount "7" @default.
• W2040343062 countsByYear W20403430622014 @default.
• W2040343062 countsByYear W20403430622017 @default.
• W2040343062 countsByYear W20403430622018 @default.
• W2040343062 countsByYear W20403430622022 @default.
• W2040343062 crossrefType "journal-article" @default.
• W2040343062 hasAuthorship W2040343062A5014115464 @default.
• W2040343062 hasAuthorship W2040343062A5068855074 @default.
• W2040343062 hasAuthorship W2040343062A5075960896 @default.
• W2040343062 hasAuthorship W2040343062A5085527072 @default.
• W2040343062 hasBestOaLocation W20403430621 @default.
• W2040343062 hasConcept C104317684 @default.
• W2040343062 hasConcept C107459253 @default.
• W2040343062 hasConcept C136302470 @default.
• W2040343062 hasConcept C145103041 @default.
• W2040343062 hasConcept C148738053 @default.
• W2040343062 hasConcept C201750760 @default.
• W2040343062 hasConcept C207168726 @default.
• W2040343062 hasConcept C207701140 @default.
• W2040343062 hasConcept C20875687 @default.
• W2040343062 hasConcept C2777164004 @default.
• W2040343062 hasConcept C2779310769 @default.
• W2040343062 hasConcept C2779329299 @default.
• W2040343062 hasConcept C2780915607 @default.
• W2040343062 hasConcept C28328180 @default.
• W2040343062 hasConcept C54355233 @default.
• W2040343062 hasConcept C86803240 @default.
• W2040343062 hasConcept C95444343 @default.
• W2040343062 hasConceptScore W2040343062C104317684 @default.
• W2040343062 hasConceptScore W2040343062C107459253 @default.
• W2040343062 hasConceptScore W2040343062C136302470 @default.
• W2040343062 hasConceptScore W2040343062C145103041 @default.
• W2040343062 hasConceptScore W2040343062C148738053 @default.
• W2040343062 hasConceptScore W2040343062C201750760 @default.
• W2040343062 hasConceptScore W2040343062C207168726 @default.
• W2040343062 hasConceptScore W2040343062C207701140 @default.
• W2040343062 hasConceptScore W2040343062C20875687 @default.
• W2040343062 hasConceptScore W2040343062C2777164004 @default.
• W2040343062 hasConceptScore W2040343062C2779310769 @default.
• W2040343062 hasConceptScore W2040343062C2779329299 @default.
• W2040343062 hasConceptScore W2040343062C2780915607 @default.
• W2040343062 hasConceptScore W2040343062C28328180 @default.
• W2040343062 hasConceptScore W2040343062C54355233 @default.
• W2040343062 hasConceptScore W2040343062C86803240 @default.
• W2040343062 hasConceptScore W2040343062C95444343 @default.
• W2040343062 hasIssue "12" @default.
• W2040343062 hasLocation W20403430621 @default.
• W2040343062 hasLocation W20403430622 @default.
• W2040343062 hasLocation W20403430623 @default.
• W2040343062 hasLocation W20403430624 @default.
• W2040343062 hasOpenAccess W2040343062 @default.
• W2040343062 hasPrimaryLocation W20403430621 @default.
• W2040343062 hasRelatedWork W1203270232 @default.
• W2040343062 hasRelatedWork W1978560791 @default.
• W2040343062 hasRelatedWork W2009519731 @default.
• W2040343062 hasRelatedWork W2040343062 @default.

• next