Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040345057> ?p ?o ?g. }
- W2040345057 endingPage "3570" @default.
- W2040345057 startingPage "3567" @default.
- W2040345057 abstract "We describe the construction of a soluble protein carrying the N-terminal extracellular domain (ECD) of the alpha7 subunit of the nicotinic acetylcholine receptor. The approach was to fuse the alpha7 ECD at the C and N termini of several monomeric and pentameric soluble carrier proteins and to investigate the soluble expression of the product in Escherichia coli. An initial screening of six carrier proteins resulted in the selection of a fusion protein comprising, from the N to the C terminus, the maltose binding protein, a 17-aa linker containing an enterokinase binding site, and the alpha7 ECD. This protein is soluble upon expression in bacteria and is purified by affinity chromatography. It binds the competitive nicotinic antagonist alpha-bungarotoxin with 2.5 microM affinity and displays a CD spectrum corresponding to a folded protein. The method might be suitable to produce large quantities of protein for crystallization and immunochemical experiments." @default.
- W2040345057 created "2016-06-24" @default.
- W2040345057 creator A5002550752 @default.
- W2040345057 creator A5006304159 @default.
- W2040345057 creator A5056074308 @default.
- W2040345057 creator A5068798153 @default.
- W2040345057 creator A5072727717 @default.
- W2040345057 date "2001-03-13" @default.
- W2040345057 modified "2023-09-24" @default.
- W2040345057 title "A method for soluble overexpression of the α7 nicotinic acetylcholine receptor extracellular domain" @default.
- W2040345057 cites W188468884 @default.
- W2040345057 cites W1964643611 @default.
- W2040345057 cites W1966766357 @default.
- W2040345057 cites W1973957927 @default.
- W2040345057 cites W1997180556 @default.
- W2040345057 cites W2000746761 @default.
- W2040345057 cites W2005579644 @default.
- W2040345057 cites W2011622844 @default.
- W2040345057 cites W2015066232 @default.
- W2040345057 cites W2018289835 @default.
- W2040345057 cites W2039111860 @default.
- W2040345057 cites W2040249970 @default.
- W2040345057 cites W2041004635 @default.
- W2040345057 cites W2051103013 @default.
- W2040345057 cites W2057551724 @default.
- W2040345057 cites W2085904210 @default.
- W2040345057 cites W2099067440 @default.
- W2040345057 cites W2100837269 @default.
- W2040345057 cites W2103770311 @default.
- W2040345057 cites W2111622691 @default.
- W2040345057 cites W2138270253 @default.
- W2040345057 cites W2166674578 @default.
- W2040345057 cites W2425257194 @default.
- W2040345057 cites W322095649 @default.
- W2040345057 doi "https://doi.org/10.1073/pnas.041594798" @default.
- W2040345057 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/30693" @default.
- W2040345057 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11248118" @default.
- W2040345057 hasPublicationYear "2001" @default.
- W2040345057 type Work @default.
- W2040345057 sameAs 2040345057 @default.
- W2040345057 citedByCount "20" @default.
- W2040345057 countsByYear W20403450572018 @default.
- W2040345057 countsByYear W20403450572019 @default.
- W2040345057 countsByYear W20403450572020 @default.
- W2040345057 crossrefType "journal-article" @default.
- W2040345057 hasAuthorship W2040345057A5002550752 @default.
- W2040345057 hasAuthorship W2040345057A5006304159 @default.
- W2040345057 hasAuthorship W2040345057A5056074308 @default.
- W2040345057 hasAuthorship W2040345057A5068798153 @default.
- W2040345057 hasAuthorship W2040345057A5072727717 @default.
- W2040345057 hasBestOaLocation W20403450571 @default.
- W2040345057 hasConcept C104292427 @default.
- W2040345057 hasConcept C104317684 @default.
- W2040345057 hasConcept C123894998 @default.
- W2040345057 hasConcept C145508813 @default.
- W2040345057 hasConcept C170493617 @default.
- W2040345057 hasConcept C179247698 @default.
- W2040345057 hasConcept C181199279 @default.
- W2040345057 hasConcept C185592680 @default.
- W2040345057 hasConcept C188987157 @default.
- W2040345057 hasConcept C19876734 @default.
- W2040345057 hasConcept C2779570518 @default.
- W2040345057 hasConcept C2780676925 @default.
- W2040345057 hasConcept C28406088 @default.
- W2040345057 hasConcept C40767141 @default.
- W2040345057 hasConcept C547475151 @default.
- W2040345057 hasConcept C55493867 @default.
- W2040345057 hasConcept C80161118 @default.
- W2040345057 hasConceptScore W2040345057C104292427 @default.
- W2040345057 hasConceptScore W2040345057C104317684 @default.
- W2040345057 hasConceptScore W2040345057C123894998 @default.
- W2040345057 hasConceptScore W2040345057C145508813 @default.
- W2040345057 hasConceptScore W2040345057C170493617 @default.
- W2040345057 hasConceptScore W2040345057C179247698 @default.
- W2040345057 hasConceptScore W2040345057C181199279 @default.
- W2040345057 hasConceptScore W2040345057C185592680 @default.
- W2040345057 hasConceptScore W2040345057C188987157 @default.
- W2040345057 hasConceptScore W2040345057C19876734 @default.
- W2040345057 hasConceptScore W2040345057C2779570518 @default.
- W2040345057 hasConceptScore W2040345057C2780676925 @default.
- W2040345057 hasConceptScore W2040345057C28406088 @default.
- W2040345057 hasConceptScore W2040345057C40767141 @default.
- W2040345057 hasConceptScore W2040345057C547475151 @default.
- W2040345057 hasConceptScore W2040345057C55493867 @default.
- W2040345057 hasConceptScore W2040345057C80161118 @default.
- W2040345057 hasIssue "6" @default.
- W2040345057 hasLocation W20403450571 @default.
- W2040345057 hasLocation W20403450572 @default.
- W2040345057 hasLocation W20403450573 @default.
- W2040345057 hasLocation W20403450574 @default.
- W2040345057 hasOpenAccess W2040345057 @default.
- W2040345057 hasPrimaryLocation W20403450571 @default.
- W2040345057 hasRelatedWork W1785917080 @default.
- W2040345057 hasRelatedWork W1965117609 @default.
- W2040345057 hasRelatedWork W2040345057 @default.
- W2040345057 hasRelatedWork W2084325489 @default.
- W2040345057 hasRelatedWork W2086988617 @default.
- W2040345057 hasRelatedWork W2228404999 @default.