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- W2040347730 abstract "We studied the effects of fluorescent labeling on the isoelectric points (pI values) of proteins using capillary isoelectric focusing with laser-induced fluorescence detection (cIEF–LIF). Specifically, we labeled green fluorescent protein (GFP) from the jellyfish Aequorea victoria with the fluorogenic dye 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ). cIEF–LIF was used to monitor the native fluorescence of GFP and showed pI changes in GFP’s FQ-labeled products. Multiple labeling of GFP with FQ produced a series of products with pI values shifted towards a low pH. We verified cIEF–LIF results with traditional slab gel IEF. Our cIEF–LIF technique can routinely detect 10−11 M of FQ-labeled protein, whereas traditional slab gel IEF with silver stain detection gives detection limits of 10−7 M in the same samples." @default.
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- W2040347730 date "1999-08-01" @default.
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- W2040347730 title "Labeling effects on the isoelectric point of green fluorescent protein" @default.
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- W2040347730 doi "https://doi.org/10.1016/s0021-9673(99)00687-1" @default.
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