FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040354421> ?p ?o ?g. }

• W2040354421 endingPage "e24173" @default.
• W2040354421 startingPage "e24173" @default.
• W2040354421 abstract "The ethylene receptor ethylene response 1 (ETR1) and the Arabidopsis histidine-containing phosphotransfer protein 1 (AHP1) form a tight complex in vitro. According to our current model ETR1 and AHP1 together with a response regulator form a phosphorelay system controlling the gene expression response to the plant hormone ethylene, similar to the two-component signaling in bacteria. The model implies that ETR1 functions as a sensor kinase and is autophosphorylated in the absence of ethylene. The phosphoryl group is then transferred onto a histidine at the canonical phosphorylation site in AHP1. For phosphoryl group transfer both binding partners need to form a tight complex. After ethylene binding the receptor is switched to the non-phosphorylated state. This switch is accompanied by a conformational change that decreases the affinity to the phosphorylated AHP1. To test this model we used fluorescence polarization and examined how the phosphorylation status of the proteins affects formation of the suggested ETR1-AHP1 signaling complex. We have employed various mutants of ETR1 and AHP1 mimicking permanent phosphorylation or preventing phosphorylation, respectively. Our results show that phosphorylation plays an important role in complex formation as affinity is dramatically reduced when the signaling partners are either both in their non-phosphorylated form or both in their phosphorylated form. On the other hand, affinity is greatly enhanced when either protein is in the phosphorylated state and the corresponding partner in its non-phosphorylated form. Our results indicate that interaction of ETR1 and AHP1 requires that ETR1 is a dimer, as in its functional state as receptor in planta." @default.
• W2040354421 created "2016-06-24" @default.
• W2040354421 creator A5021507772 @default.
• W2040354421 creator A5068338059 @default.
• W2040354421 date "2011-09-02" @default.
• W2040354421 modified "2023-10-15" @default.
• W2040354421 title "Phosphorylation Alters the Interaction of the Arabidopsis Phosphotransfer Protein AHP1 with Its Sensor Kinase ETR1" @default.
• W2040354421 cites W1972853432 @default.
• W2040354421 cites W1980321600 @default.
• W2040354421 cites W1986926047 @default.
• W2040354421 cites W1993645665 @default.
• W2040354421 cites W1997820716 @default.
• W2040354421 cites W1998148935 @default.
• W2040354421 cites W2003173053 @default.
• W2040354421 cites W2014090222 @default.
• W2040354421 cites W2017059959 @default.
• W2040354421 cites W2029582401 @default.
• W2040354421 cites W2047399603 @default.
• W2040354421 cites W2048661284 @default.
• W2040354421 cites W2051450127 @default.
• W2040354421 cites W2052126555 @default.
• W2040354421 cites W2052880006 @default.
• W2040354421 cites W2054292089 @default.
• W2040354421 cites W2055326419 @default.
• W2040354421 cites W2055410211 @default.
• W2040354421 cites W2061673106 @default.
• W2040354421 cites W2065283382 @default.
• W2040354421 cites W2067334188 @default.
• W2040354421 cites W2069713760 @default.
• W2040354421 cites W2078941883 @default.
• W2040354421 cites W2079444950 @default.
• W2040354421 cites W2085481693 @default.
• W2040354421 cites W2087025822 @default.
• W2040354421 cites W2087146475 @default.
• W2040354421 cites W2087444181 @default.
• W2040354421 cites W2091361840 @default.
• W2040354421 cites W2094093931 @default.
• W2040354421 cites W2095704882 @default.
• W2040354421 cites W2096137680 @default.
• W2040354421 cites W2101871915 @default.
• W2040354421 cites W2104314673 @default.
• W2040354421 cites W2108559568 @default.
• W2040354421 cites W2112352925 @default.
• W2040354421 cites W2113263286 @default.
• W2040354421 cites W2115438572 @default.
• W2040354421 cites W2138026076 @default.
• W2040354421 cites W2141947281 @default.
• W2040354421 cites W2145936558 @default.
• W2040354421 cites W2156206497 @default.
• W2040354421 cites W2160259646 @default.
• W2040354421 cites W4245067683 @default.
• W2040354421 doi "https://doi.org/10.1371/journal.pone.0024173" @default.
• W2040354421 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3166298" @default.
• W2040354421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21912672" @default.
• W2040354421 hasPublicationYear "2011" @default.
• W2040354421 type Work @default.
• W2040354421 sameAs 2040354421 @default.
• W2040354421 citedByCount "35" @default.
• W2040354421 countsByYear W20403544212012 @default.
• W2040354421 countsByYear W20403544212013 @default.
• W2040354421 countsByYear W20403544212014 @default.
• W2040354421 countsByYear W20403544212015 @default.
• W2040354421 countsByYear W20403544212016 @default.
• W2040354421 countsByYear W20403544212017 @default.
• W2040354421 countsByYear W20403544212018 @default.
• W2040354421 countsByYear W20403544212019 @default.
• W2040354421 countsByYear W20403544212020 @default.
• W2040354421 countsByYear W20403544212021 @default.
• W2040354421 countsByYear W20403544212022 @default.
• W2040354421 countsByYear W20403544212023 @default.
• W2040354421 crossrefType "journal-article" @default.
• W2040354421 hasAuthorship W2040354421A5021507772 @default.
• W2040354421 hasAuthorship W2040354421A5068338059 @default.
• W2040354421 hasBestOaLocation W20403544211 @default.
• W2040354421 hasConcept C104317684 @default.
• W2040354421 hasConcept C104551158 @default.
• W2040354421 hasConcept C11960822 @default.
• W2040354421 hasConcept C138223613 @default.
• W2040354421 hasConcept C143065580 @default.
• W2040354421 hasConcept C181199279 @default.
• W2040354421 hasConcept C184235292 @default.
• W2040354421 hasConcept C185592680 @default.
• W2040354421 hasConcept C2778460671 @default.
• W2040354421 hasConcept C2778905257 @default.
• W2040354421 hasConcept C2779491563 @default.
• W2040354421 hasConcept C2781196521 @default.
• W2040354421 hasConcept C55493867 @default.
• W2040354421 hasConcept C62478195 @default.
• W2040354421 hasConcept C86803240 @default.
• W2040354421 hasConcept C87325107 @default.
• W2040354421 hasConcept C95444343 @default.
• W2040354421 hasConcept C97029542 @default.
• W2040354421 hasConceptScore W2040354421C104317684 @default.
• W2040354421 hasConceptScore W2040354421C104551158 @default.
• W2040354421 hasConceptScore W2040354421C11960822 @default.
• W2040354421 hasConceptScore W2040354421C138223613 @default.
• W2040354421 hasConceptScore W2040354421C143065580 @default.
• W2040354421 hasConceptScore W2040354421C181199279 @default.

• next