FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040367407> ?p ?o ?g. }

• W2040367407 abstract "Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. Indeed, nearly 40% of genes in plants encode proteins of unknown function. Functional characterization of these genes is one of the main challenges in modern biology. In this regard, the availability of full-length cDNA clones may fill in the gap created between sequence information and biological knowledge. Full-length cDNA clones facilitate functional analysis of the corresponding genes enabling manipulation of their expression in heterologous systems and the generation of a variety of tagged versions of the native protein. In addition, the development of full-length cDNA sequences has the power to improve the quality of genome annotation.We developed an integrated method to generate a new normalized EST collection enriched in full-length and rare transcripts of different citrus species from multiple tissues and developmental stages. We constructed a total of 15 cDNA libraries, from which we isolated 10,898 high-quality ESTs representing 6142 different genes. Percentages of redundancy and proportion of full-length clones range from 8 to 33, and 67 to 85, respectively, indicating good efficiency of the approach employed. The new EST collection adds 2113 new citrus ESTs, representing 1831 unigenes, to the collection of citrus genes available in the public databases. To facilitate functional analysis, cDNAs were introduced in a Gateway-based cloning vector for high-throughput functional analysis of genes in planta. Herein, we describe the technical methods used in the library construction, sequence analysis of clones and the overexpression of CitrSEP, a citrus homolog to the Arabidopsis SEP3 gene, in Arabidopsis as an example of a practical application of the engineered Gateway vector for functional analysis.The new EST collection denotes an important step towards the identification of all genes in the citrus genome. Furthermore, public availability of the cDNA clones generated in this study, and not only their sequence, enables testing of the biological function of the genes represented in the collection. Expression of the citrus SEP3 homologue, CitrSEP, in Arabidopsis results in early flowering, along with other phenotypes resembling the over-expression of the Arabidopsis SEPALLATA genes. Our findings suggest that the members of the SEP gene family play similar roles in these quite distant plant species." @default.
• W2040367407 created "2016-06-24" @default.
• W2040367407 creator A5004480334 @default.
• W2040367407 creator A5018375771 @default.
• W2040367407 creator A5019167224 @default.
• W2040367407 creator A5051218241 @default.
• W2040367407 creator A5066331391 @default.
• W2040367407 creator A5075382335 @default.
• W2040367407 creator A5086526598 @default.
• W2040367407 date "2009-09-11" @default.
• W2040367407 modified "2023-10-18" @default.
• W2040367407 title "A new set of ESTs and cDNA clones from full-length and normalized libraries for gene discovery and functional characterization in citrus" @default.
• W2040367407 cites W1890245273 @default.
• W2040367407 cites W1949623853 @default.
• W2040367407 cites W1973931828 @default.
• W2040367407 cites W1981012177 @default.
• W2040367407 cites W1985726486 @default.
• W2040367407 cites W1986508375 @default.
• W2040367407 cites W1994470176 @default.
• W2040367407 cites W1995775282 @default.
• W2040367407 cites W1997438772 @default.
• W2040367407 cites W1998415512 @default.
• W2040367407 cites W1998746328 @default.
• W2040367407 cites W2003870484 @default.
• W2040367407 cites W2030311040 @default.
• W2040367407 cites W2033792606 @default.
• W2040367407 cites W2034219945 @default.
• W2040367407 cites W2046944468 @default.
• W2040367407 cites W2052184218 @default.
• W2040367407 cites W2060687391 @default.
• W2040367407 cites W2060810338 @default.
• W2040367407 cites W2064395884 @default.
• W2040367407 cites W2074242397 @default.
• W2040367407 cites W2082495866 @default.
• W2040367407 cites W2083553146 @default.
• W2040367407 cites W2084847549 @default.
• W2040367407 cites W2093007889 @default.
• W2040367407 cites W2094448442 @default.
• W2040367407 cites W2096940779 @default.
• W2040367407 cites W2106214237 @default.
• W2040367407 cites W2113754798 @default.
• W2040367407 cites W2116122104 @default.
• W2040367407 cites W2121016876 @default.
• W2040367407 cites W2128925984 @default.
• W2040367407 cites W2137839422 @default.
• W2040367407 cites W2138194381 @default.
• W2040367407 cites W2141429272 @default.
• W2040367407 cites W2144905501 @default.
• W2040367407 cites W2144921226 @default.
• W2040367407 cites W2148251122 @default.
• W2040367407 cites W2150892841 @default.
• W2040367407 cites W2151831732 @default.
• W2040367407 cites W2152532695 @default.
• W2040367407 cites W2155530972 @default.
• W2040367407 cites W2161732286 @default.
• W2040367407 cites W2162731300 @default.
• W2040367407 cites W2163448850 @default.
• W2040367407 cites W2169907253 @default.
• W2040367407 cites W2576734941 @default.
• W2040367407 cites W4210400672 @default.
• W2040367407 doi "https://doi.org/10.1186/1471-2164-10-428" @default.
• W2040367407 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2754500" @default.
• W2040367407 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19747386" @default.
• W2040367407 hasPublicationYear "2009" @default.
• W2040367407 type Work @default.
• W2040367407 sameAs 2040367407 @default.
• W2040367407 citedByCount "27" @default.
• W2040367407 countsByYear W20403674072012 @default.
• W2040367407 countsByYear W20403674072013 @default.
• W2040367407 countsByYear W20403674072014 @default.
• W2040367407 countsByYear W20403674072015 @default.
• W2040367407 countsByYear W20403674072018 @default.
• W2040367407 countsByYear W20403674072019 @default.
• W2040367407 countsByYear W20403674072020 @default.
• W2040367407 countsByYear W20403674072021 @default.
• W2040367407 crossrefType "journal-article" @default.
• W2040367407 hasAuthorship W2040367407A5004480334 @default.
• W2040367407 hasAuthorship W2040367407A5018375771 @default.
• W2040367407 hasAuthorship W2040367407A5019167224 @default.
• W2040367407 hasAuthorship W2040367407A5051218241 @default.
• W2040367407 hasAuthorship W2040367407A5066331391 @default.
• W2040367407 hasAuthorship W2040367407A5075382335 @default.
• W2040367407 hasAuthorship W2040367407A5086526598 @default.
• W2040367407 hasBestOaLocation W20403674071 @default.
• W2040367407 hasConcept C104317684 @default.
• W2040367407 hasConcept C105565629 @default.
• W2040367407 hasConcept C141231307 @default.
• W2040367407 hasConcept C149034497 @default.
• W2040367407 hasConcept C150194340 @default.
• W2040367407 hasConcept C161078062 @default.
• W2040367407 hasConcept C187882448 @default.
• W2040367407 hasConcept C189206191 @default.
• W2040367407 hasConcept C189482833 @default.
• W2040367407 hasConcept C2908923196 @default.
• W2040367407 hasConcept C54355233 @default.
• W2040367407 hasConcept C61053724 @default.
• W2040367407 hasConcept C70721500 @default.
• W2040367407 hasConcept C86803240 @default.
• W2040367407 hasConcept C95371953 @default.
• W2040367407 hasConceptScore W2040367407C104317684 @default.

• next