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- W2040369626 abstract "An Hfr lac gene is transferred into a recipient lacking the lac region and selection is made for lac recombinant colonies. Mutagenic pulses are applied by nitrosoguanidine treatment, which produces point mutations in one DNA strand. Mutation of the i gene immediately after transfer results in heterozygous recombinant colonies, containing both i+ and i− segregants. Mutation of the i gene immediately before transfer gives rise only to homozygous i− recombinant colonies. This is expected if only one DNA strand is transferred, whereas transfer of double-stranded DNA mutated in one of the strands would most likely lead to heterozygous i− colonies. This finding, together with appropriate controls, therefore provides evidence that only one of the donor DNA strands enters the recipient, where it is immediately replicated." @default.
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- W2040369626 date "1968-10-01" @default.
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- W2040369626 title "Genetic evidence for transfer of a single DNA strand during bacterial conjugation" @default.
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- W2040369626 doi "https://doi.org/10.1016/0022-2836(68)90074-0" @default.
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