Matches in SemOpenAlex for { <https://semopenalex.org/work/W2040370019> ?p ?o ?g. }
- W2040370019 endingPage "204" @default.
- W2040370019 startingPage "196" @default.
- W2040370019 abstract "Visualizing overall tissue architecture in three dimensions is fundamental for validating and integrating biochemical, cell biological and visual data from less complex systems such as cultured cells. Here, we describe a method to generate high-resolution three-dimensional image data of intact mouse gut tissue. Regions of highest interest lie between 50 and 200 mum within this tissue. The quality and usefulness of three-dimensional image data of tissue with such depth is limited owing to problems associated with scattered light, photobleaching and spherical aberration. Furthermore, the highest-quality oil-immersion lenses are designed to work at a maximum distance of </=10-15 mum into the sample, further compounding the ability to image at high-resolution deep within tissue. We show that manipulating the refractive index of the mounting media and decreasing sample opacity greatly improves image quality such that the limiting factor for a standard, inverted multi-photon microscope is determined by the working distance of the objective as opposed to detectable fluorescence. This method negates the need for mechanical sectioning of tissue and enables the routine generation of high-quality, quantitative image data that can significantly advance our understanding of tissue architecture and physiology." @default.
- W2040370019 created "2016-06-24" @default.
- W2040370019 creator A5003734971 @default.
- W2040370019 creator A5048435051 @default.
- W2040370019 creator A5053386502 @default.
- W2040370019 creator A5059876417 @default.
- W2040370019 date "2009-05-01" @default.
- W2040370019 modified "2023-09-25" @default.
- W2040370019 title "Preparation of wholemount mouse intestine for high-resolution three-dimensional imaging using two-photon microscopy" @default.
- W2040370019 cites W1982603620 @default.
- W2040370019 cites W1984842461 @default.
- W2040370019 cites W1988418842 @default.
- W2040370019 cites W2000259686 @default.
- W2040370019 cites W2003299661 @default.
- W2040370019 cites W2009663935 @default.
- W2040370019 cites W2012728158 @default.
- W2040370019 cites W2014644083 @default.
- W2040370019 cites W2042194419 @default.
- W2040370019 cites W2044555293 @default.
- W2040370019 cites W2045020713 @default.
- W2040370019 cites W2048436980 @default.
- W2040370019 cites W2061464631 @default.
- W2040370019 cites W2068494191 @default.
- W2040370019 cites W2069402572 @default.
- W2040370019 cites W2081917343 @default.
- W2040370019 cites W2093570357 @default.
- W2040370019 cites W2101545913 @default.
- W2040370019 cites W2144307710 @default.
- W2040370019 cites W2147607085 @default.
- W2040370019 cites W2154083988 @default.
- W2040370019 cites W2167952334 @default.
- W2040370019 cites W2171788130 @default.
- W2040370019 doi "https://doi.org/10.1111/j.1365-2818.2009.03163.x" @default.
- W2040370019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19397748" @default.
- W2040370019 hasPublicationYear "2009" @default.
- W2040370019 type Work @default.
- W2040370019 sameAs 2040370019 @default.
- W2040370019 citedByCount "37" @default.
- W2040370019 countsByYear W20403700192012 @default.
- W2040370019 countsByYear W20403700192013 @default.
- W2040370019 countsByYear W20403700192015 @default.
- W2040370019 countsByYear W20403700192016 @default.
- W2040370019 countsByYear W20403700192017 @default.
- W2040370019 countsByYear W20403700192018 @default.
- W2040370019 countsByYear W20403700192019 @default.
- W2040370019 countsByYear W20403700192022 @default.
- W2040370019 crossrefType "journal-article" @default.
- W2040370019 hasAuthorship W2040370019A5003734971 @default.
- W2040370019 hasAuthorship W2040370019A5048435051 @default.
- W2040370019 hasAuthorship W2040370019A5053386502 @default.
- W2040370019 hasAuthorship W2040370019A5059876417 @default.
- W2040370019 hasConcept C115961682 @default.
- W2040370019 hasConcept C120665830 @default.
- W2040370019 hasConcept C121332964 @default.
- W2040370019 hasConcept C136229726 @default.
- W2040370019 hasConcept C138268822 @default.
- W2040370019 hasConcept C147080431 @default.
- W2040370019 hasConcept C154945302 @default.
- W2040370019 hasConcept C186060115 @default.
- W2040370019 hasConcept C192562407 @default.
- W2040370019 hasConcept C20446124 @default.
- W2040370019 hasConcept C205372480 @default.
- W2040370019 hasConcept C31332276 @default.
- W2040370019 hasConcept C31972630 @default.
- W2040370019 hasConcept C41008148 @default.
- W2040370019 hasConcept C47785574 @default.
- W2040370019 hasConcept C55020928 @default.
- W2040370019 hasConcept C60056205 @default.
- W2040370019 hasConcept C67649825 @default.
- W2040370019 hasConcept C71924100 @default.
- W2040370019 hasConcept C86803240 @default.
- W2040370019 hasConcept C91881484 @default.
- W2040370019 hasConceptScore W2040370019C115961682 @default.
- W2040370019 hasConceptScore W2040370019C120665830 @default.
- W2040370019 hasConceptScore W2040370019C121332964 @default.
- W2040370019 hasConceptScore W2040370019C136229726 @default.
- W2040370019 hasConceptScore W2040370019C138268822 @default.
- W2040370019 hasConceptScore W2040370019C147080431 @default.
- W2040370019 hasConceptScore W2040370019C154945302 @default.
- W2040370019 hasConceptScore W2040370019C186060115 @default.
- W2040370019 hasConceptScore W2040370019C192562407 @default.
- W2040370019 hasConceptScore W2040370019C20446124 @default.
- W2040370019 hasConceptScore W2040370019C205372480 @default.
- W2040370019 hasConceptScore W2040370019C31332276 @default.
- W2040370019 hasConceptScore W2040370019C31972630 @default.
- W2040370019 hasConceptScore W2040370019C41008148 @default.
- W2040370019 hasConceptScore W2040370019C47785574 @default.
- W2040370019 hasConceptScore W2040370019C55020928 @default.
- W2040370019 hasConceptScore W2040370019C60056205 @default.
- W2040370019 hasConceptScore W2040370019C67649825 @default.
- W2040370019 hasConceptScore W2040370019C71924100 @default.
- W2040370019 hasConceptScore W2040370019C86803240 @default.
- W2040370019 hasConceptScore W2040370019C91881484 @default.
- W2040370019 hasIssue "2" @default.
- W2040370019 hasLocation W20403700191 @default.
- W2040370019 hasLocation W20403700192 @default.
- W2040370019 hasOpenAccess W2040370019 @default.
- W2040370019 hasPrimaryLocation W20403700191 @default.